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    The Social-Ecological Systems (SES) framework serves as a valuable framework to explore and understand social and ecological interactions, and pathways in water governance. Yet, it lacks a robust understanding of change. We argue an analytical and methodological approach to engaging global changes in SES is critical to strengthening the scope and relevance of the SES framework. Relying on SES and resilience thinking, we propose an institutional and cognitive model of change that institutions and natural resources systems co-evolve to provide a dynamic understanding of SES that stands on three causal mechanisms: institutional complexity trap, rigidity trap, and learning processes. We illustrate how Data Cube technology could overcome current limitations and offer reliable avenues to test hypothesis about the dynamics of social-ecological systems and water security by offering to combine spatial and time data with no major technical requirements for users.

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    https://doi.org/10.20944/prepr...
    Article . 2018 . Peer-reviewed
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    Sustainability
    Article . 2018 . Peer-reviewed
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    https://doi.org/10.20944/prepr...
    Article . 2018 . Peer-reviewed
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ https://doi.org/10.2...arrow_drop_down
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      Article . 2018 . Peer-reviewed
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    Authors: Ismail Adal Guiamel; Han Soo Lee;

    This study aims to identify potential hydropower sites and calculate the theoretical potential hydropower capacity based on watershed modelling of the Mindanao River Basin (MRB) in the Philippines for the sustainable development of a previously unstudied region. The Soil and Water Assessment Tool (SWAT) was applied to delineate the watershed of the MRB and simulate the river discharges with inputs from observed precipitation and global gridded precipitation datasets. Observed weather data, such as temperature, humidity, and solar radiation, from four meteorological stations in the Philippines were also used as inputs for SWAT modelling. Simulated discharges were calibrated at three river gauges on the Nituan, Libungan and Pulangi Rivers. However, due to limited river discharge records, model validations were conducted in proxy basins: the calibrated model parameters in river A were used in the watershed modelling of proxy river B. Of the delineated 107 sub-basins in the MRB watershed, only 33 were identified as having potential sites for hydropower development. These potential sub-basins hosted a total of 154 potential sites with an estimated monthly average power capacity of 5,551.35 MW for all 33 sub-basins. The estimated theoretical power capacity of 15,266.22 MW for all sites in the MRB is approximately equivalent to the Philippines’ total available power capacity in 2017 of 15,393 MW. These sites were classified into 16 mini-scale hydropower sites, 114 small-scale hydropower sites and 24 medium-scale hydropower sites based on the simulated river discharges and potential power capacities. Based on these results, hydropower development could be an alternative to strengthen the exploration of renewable energy resources and improve the energy situation in Mindanao; hydropower development could also have mitigation effects on frequent floods in flat, low-lying downstream areas.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Energy Reportsarrow_drop_down
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    Energy Reports
    Article . 2020 . Peer-reviewed
    License: CC BY
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    Energy Reports
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    Energy Reports
    Article . 2020
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      Energy Reports
      Article . 2020 . Peer-reviewed
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      Energy Reports
      Article . 2020
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    Authors: Toshihiro Takahashi; Kazuo Nakayama;

    AbstractA simple experiment for capillary sealed trap shows that leakage would occur only from the weakest point in the trap. As an extens ion of this concept, the sequestrated and accumulated CO2 in the structural high may happen to leak from the unexpected weak point near the top of sealing layer. On the other hand, if CO2 were sequestrated into the flank of tilted aquifer, CO2 in separate phas e would migrate upward within aquifer with no CO2 leakage into the sealing layer above and leaving some residual amount behind. According to this concept, the best location for sequestration of CO2 could be the flank of the structure rather than the top.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Energy Procediaarrow_drop_down
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    Energy Procedia
    Article . 2009 . Peer-reviewed
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    Energy Procedia
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      Energy Procedia
      Article . 2009 . Peer-reviewed
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    Authors: Heath, L.; Salinger, M. J.; Falkland, T.; Hansen, J.; +9 Authors

    The impacts of increasing natural climate disasters are threatening food security in the Asia-Pacific region. Rice is Asia’s most important staple food. Climate variability and change directly impact rice production, through changes in rainfall, temperature and CO2 concentrations. The key for sustainable rice crop is water management. Adaptation can occur through shifts of cropping to higher latitudes and can profit from river systems (via irrigation) so far not considered. New opportunities arise to produce more than one crop per year in cooler areas. Asian wheat production in 2005 represents about 43 % of the global total. Changes in agronomic practices, such as earlier plant dates and cultivar substitution will be required. Fisheries play a crucial role in providing food security with the contribution of fish to dietary animal protein being very high in the region – up to 90 % in small island developing states (SIDS). With the warming of the Pacific and Indian Oceans and increased acidification, marine ecosystems are presently under stress. Despite these trends, maintaining or enhancing food production from the sea is critical. However, future sustainability must be maintained whilst also securing biodiversity conservation. Improved fisheries management to address the existing non-climate threats remains paramount in the Indian and Pacific Oceans with sustainable management regimes being established. Climate-related impacts are expected to increase in magnitude over the coming decades, thus preliminary adaptation to climate change is valuable.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ CGIAR CGSpace (Consu...arrow_drop_down
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    https://doi.org/10.1007/978-94...
    Part of book or chapter of book . 2013 . Peer-reviewed
    License: Springer Nature TDM
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      https://doi.org/10.1007/978-94...
      Part of book or chapter of book . 2013 . Peer-reviewed
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    Authors: null Neha; Gajender Yadav; Rajender Kumar Yadav; Ashwani Kumar; +4 Authors

    Soil salinity and the use of saline groundwater are two major constraints in crop production, which covers a ~1.0 billion ha area of arid and semi-arid regions. The improved drainage function of soil can modify the salty growing environment for higher agricultural production. The present study evaluated the effectiveness of cut-soiler-constructed rice residue-filled preferential shallow subsurface drainage (PSSD) to improve the drainage function and its effect on the yield, quality and plant–water relations of mustard over 2019–2021. Cut-soiler-simulated drains were made in a semi-controlled lysimeter (2 × 2 × 3; L*W*H m) as the main plot treatment in a double replicated split–split experiment with two soil types (subplot) and three irrigation water salinities (4, 8 and 12 dS m−1) as the sub-sub-plot treatment. The drainage volume of variable salinity (EC), dependent on the total water input, was substantially higher in the rainy season (April to October), i.e., 16.6, 7.76 and 12.0% during 2018, 2019 and 2020, with 1.7, 0.32 and 0.77 kg salt removal per lysimeter, compared to the post-rainy season. The mustard seed, straw and biological yields were improved by 31.4, 14.41 and 18.08%, respectively, due to a positive effect on plant–water relations. The mustard seeds produced in the cut-soiler-treated plots recorded higher oil, crude fiber and protein contents and a lower erucic acid content. The increase in salt load, by higher-salinity irrigation water, was also efficiently managed by using cut-soiler PSSD. It was found that the saline irrigation water up to 12.0 dS m−1 can be used under such PSSD without any extra salt loading. The present study showed the potential of cut-soiler PSSD in root zone salinity management by improving drainage in salt-affected arid regions.

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    Sustainability
    Article . 2022 . Peer-reviewed
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    Authors: Ayushi Gaur; Abhishek Gaur; Dai Yamazaki; Slobodan P. Simonovic;

    This study discusses the flooding related consequences of climate change on most populous Canadian cities and flow regulation infrastructure (FRI). The discussion is based on the aggregated results of historical and projected future flooding frequencies and flood timing as generated by Canada-wide hydrodynamic modelling in a previous study. Impact assessment on 100 most populous Canadian cities indicate that future flooding frequencies in some of the most populous cities such as Toronto and Montreal can be expected to increase from 100 (250) years to 15 (22) years by the end of the 21st century making these cities highest at risk to projected changes in flooding frequencies as a consequence of climate change. Overall 40–60% of the analyzed cities are found to be associated with future increases in flooding frequencies and associated increases in flood hazard and flood risk. The flooding related impacts of climate change on 1072 FRIs located across Canada are assessed both in terms of projected changes in future flooding frequencies and changes in flood timings. Results suggest that 40–50% of the FRIs especially those located in southern Ontario, western coastal regions, and northern regions of Canada can be expected to experience future increases in flooding frequencies. FRIs located in many of these regions are also projected to experience future changes in flood timing underlining that operating rules for those FRIs may need to be reassessed to make them resilient to changing climate.

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    Authors: Kenkichi Ishigure;

    Nuclear power generation is now one of the most important technologies in the energy supply in Japan. The operational experience of nuclear power plants for more than 20 years in Japan has shown that water chemistry is a key technology for safer and more economical operation of nuclear power plants. Extensive efforts have been made in the field of water chemistry to achieve the targets of radiation field control, to keep the integrities of piping and fuel elements and to reduce the radioactive waste generation. This paper briefly describes the present status of Japanese water chemistry technology with the main emphasis placed on BWRs.

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    Nuclear Engineering and Design
    Article . 1996 . Peer-reviewed
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      Nuclear Engineering and Design
      Article . 1996 . Peer-reviewed
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    Authors: Yoshitaka Sakata; Takao Katsura; Ahmed A. Serageldin; Katsunori Nagano; +1 Authors

    The variability of ground thermal conductivity, based on underground conditions, is often ignored during the design of ground-source heat pump systems. This study shows a field evidence of such site-scale variations through thermal response tests in eight borehole heat exchangers aligned at a site on a terrace along the foothills of mountains in northern Japan. Conventional analysis of the overall ground thermal conductivity along the total installation length finds that the value at one borehole heat exchanger is 2.5 times that at the other seven boreholes. History matching analysis of underground distributed temperature measurements generates vertical partial ground thermal conductivity data for four depth layers. Based on the moving line heat source theory, the partial values are generally within a narrow range expected for gravel deposits. Darcy velocities of groundwater are estimated to be 74–204 m/y at the borehole with high conductivity, increasing in the shallow layers above a depth of 41 m. In contrast, the velocities at the other seven boreholes are one-to-two orders of magnitude smaller with no trend. These high and low velocity values are considered for the topography and permeability. However, the relatively slow groundwater velocities might not apparently increase the partial conductivity.

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    Energies
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    Authors: M. Venkateswar Reddy; M. Venkateswar Reddy; Young-Cheol Chang;

    Medium-chain fatty acids (MCFA) such as caproic, heptanoic, and caprylic acids are monocarboxylic acids, which can be used as precursor molecules to synthesize biodiesel, bioplastics, antimicrobials, and corrosion inhibitors.

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    Sustainable Energy & Fuels
    Article . 2021 . Peer-reviewed
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      Sustainable Energy & Fuels
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    Authors: Ryota Koga; Miho Kitayama; Atsushi Kouzuma; Kazuya Watanabe; +1 Authors

    ABSTRACT An electrochemical flow cell equipped with a graphite working electrode (WE) at the bottom was inoculated with Shewanella oneidensis MR-1 expressing an anaerobic fluorescent protein, and biofilm formation on the WE was observed over time during current generation at WE potentials of +0.4 and 0 V (versus standard hydrogen electrodes), under electrolyte-flow conditions. Electrochemical analyses suggested the presence of unique electron-transfer mechanisms in the +0.4-V biofilm. Microscopic analyses revealed that, in contrast to aerobic biofilms, current-generating biofilm (at +0.4 V) was thin and flat (∼10 μm in thickness), and cells were evenly and densely distributed in the biofilm. In contrast, cells were unevenly distributed in biofilm formed at 0 V. In situ fluorescence staining and biofilm recovery experiments showed that the amounts of extracellular polysaccharides (EPSs) in the +0.4-V biofilm were much smaller than those in the aerobic and 0-V biofilms, suggesting that Shewanella cells suppress the production of EPSs at +0.4 V under flow conditions. We suggest that Shewanella cells perceive electrode potentials and modulate the structure and composition of biofilms to efficiently transfer electrons to electrodes. IMPORTANCE A promising application of microbial fuel cells (MFCs) is to save energy in wastewater treatment. Since current is generated in these MFCs by biofilm microbes under horizontal flows of wastewater, it is important to understand the mechanisms for biofilm formation and current generation under water-flow conditions. Although massive work has been done to analyze the molecular mechanisms for current generation by model exoelectrogenic bacteria, such as Shewanella oneidensis , limited information is available regarding the formation of current-generating biofilms over time under water-flow conditions. The present study developed electrochemical flow cells and used them to examine the electrochemical and structural features of current-generating biofilms under water-flow conditions. We show unique features of mature biofilms actively generating current, creating opportunities to search for as-yet-undiscovered current-generating mechanisms in Shewanella biofilms. Furthermore, information provided in the present study is useful for researchers attempting to develop anode architectures suitable for wastewater treatment MFCs.

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    Applied and Environmental Microbiology
    Article . 2017 . Peer-reviewed
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      Applied and Environmental Microbiology
      Article . 2017 . Peer-reviewed
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    The Social-Ecological Systems (SES) framework serves as a valuable framework to explore and understand social and ecological interactions, and pathways in water governance. Yet, it lacks a robust understanding of change. We argue an analytical and methodological approach to engaging global changes in SES is critical to strengthening the scope and relevance of the SES framework. Relying on SES and resilience thinking, we propose an institutional and cognitive model of change that institutions and natural resources systems co-evolve to provide a dynamic understanding of SES that stands on three causal mechanisms: institutional complexity trap, rigidity trap, and learning processes. We illustrate how Data Cube technology could overcome current limitations and offer reliable avenues to test hypothesis about the dynamics of social-ecological systems and water security by offering to combine spatial and time data with no major technical requirements for users.

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    https://doi.org/10.20944/prepr...
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    Sustainability
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    Authors: Ismail Adal Guiamel; Han Soo Lee;

    This study aims to identify potential hydropower sites and calculate the theoretical potential hydropower capacity based on watershed modelling of the Mindanao River Basin (MRB) in the Philippines for the sustainable development of a previously unstudied region. The Soil and Water Assessment Tool (SWAT) was applied to delineate the watershed of the MRB and simulate the river discharges with inputs from observed precipitation and global gridded precipitation datasets. Observed weather data, such as temperature, humidity, and solar radiation, from four meteorological stations in the Philippines were also used as inputs for SWAT modelling. Simulated discharges were calibrated at three river gauges on the Nituan, Libungan and Pulangi Rivers. However, due to limited river discharge records, model validations were conducted in proxy basins: the calibrated model parameters in river A were used in the watershed modelling of proxy river B. Of the delineated 107 sub-basins in the MRB watershed, only 33 were identified as having potential sites for hydropower development. These potential sub-basins hosted a total of 154 potential sites with an estimated monthly average power capacity of 5,551.35 MW for all 33 sub-basins. The estimated theoretical power capacity of 15,266.22 MW for all sites in the MRB is approximately equivalent to the Philippines’ total available power capacity in 2017 of 15,393 MW. These sites were classified into 16 mini-scale hydropower sites, 114 small-scale hydropower sites and 24 medium-scale hydropower sites based on the simulated river discharges and potential power capacities. Based on these results, hydropower development could be an alternative to strengthen the exploration of renewable energy resources and improve the energy situation in Mindanao; hydropower development could also have mitigation effects on frequent floods in flat, low-lying downstream areas.

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    Energy Reports
    Article . 2020 . Peer-reviewed
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    Energy Reports
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      Energy Reports
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    Authors: Toshihiro Takahashi; Kazuo Nakayama;

    AbstractA simple experiment for capillary sealed trap shows that leakage would occur only from the weakest point in the trap. As an extens ion of this concept, the sequestrated and accumulated CO2 in the structural high may happen to leak from the unexpected weak point near the top of sealing layer. On the other hand, if CO2 were sequestrated into the flank of tilted aquifer, CO2 in separate phas e would migrate upward within aquifer with no CO2 leakage into the sealing layer above and leaving some residual amount behind. According to this concept, the best location for sequestration of CO2 could be the flank of the structure rather than the top.

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    Energy Procedia
    Article . 2009 . Peer-reviewed
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    Authors: Heath, L.; Salinger, M. J.; Falkland, T.; Hansen, J.; +9 Authors

    The impacts of increasing natural climate disasters are threatening food security in the Asia-Pacific region. Rice is Asia’s most important staple food. Climate variability and change directly impact rice production, through changes in rainfall, temperature and CO2 concentrations. The key for sustainable rice crop is water management. Adaptation can occur through shifts of cropping to higher latitudes and can profit from river systems (via irrigation) so far not considered. New opportunities arise to produce more than one crop per year in cooler areas. Asian wheat production in 2005 represents about 43 % of the global total. Changes in agronomic practices, such as earlier plant dates and cultivar substitution will be required. Fisheries play a crucial role in providing food security with the contribution of fish to dietary animal protein being very high in the region – up to 90 % in small island developing states (SIDS). With the warming of the Pacific and Indian Oceans and increased acidification, marine ecosystems are presently under stress. Despite these trends, maintaining or enhancing food production from the sea is critical. However, future sustainability must be maintained whilst also securing biodiversity conservation. Improved fisheries management to address the existing non-climate threats remains paramount in the Indian and Pacific Oceans with sustainable management regimes being established. Climate-related impacts are expected to increase in magnitude over the coming decades, thus preliminary adaptation to climate change is valuable.

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    https://doi.org/10.1007/978-94...
    Part of book or chapter of book . 2013 . Peer-reviewed
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      https://doi.org/10.1007/978-94...
      Part of book or chapter of book . 2013 . Peer-reviewed
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    Authors: null Neha; Gajender Yadav; Rajender Kumar Yadav; Ashwani Kumar; +4 Authors

    Soil salinity and the use of saline groundwater are two major constraints in crop production, which covers a ~1.0 billion ha area of arid and semi-arid regions. The improved drainage function of soil can modify the salty growing environment for higher agricultural production. The present study evaluated the effectiveness of cut-soiler-constructed rice residue-filled preferential shallow subsurface drainage (PSSD) to improve the drainage function and its effect on the yield, quality and plant–water relations of mustard over 2019–2021. Cut-soiler-simulated drains were made in a semi-controlled lysimeter (2 × 2 × 3; L*W*H m) as the main plot treatment in a double replicated split–split experiment with two soil types (subplot) and three irrigation water salinities (4, 8 and 12 dS m−1) as the sub-sub-plot treatment. The drainage volume of variable salinity (EC), dependent on the total water input, was substantially higher in the rainy season (April to October), i.e., 16.6, 7.76 and 12.0% during 2018, 2019 and 2020, with 1.7, 0.32 and 0.77 kg salt removal per lysimeter, compared to the post-rainy season. The mustard seed, straw and biological yields were improved by 31.4, 14.41 and 18.08%, respectively, due to a positive effect on plant–water relations. The mustard seeds produced in the cut-soiler-treated plots recorded higher oil, crude fiber and protein contents and a lower erucic acid content. The increase in salt load, by higher-salinity irrigation water, was also efficiently managed by using cut-soiler PSSD. It was found that the saline irrigation water up to 12.0 dS m−1 can be used under such PSSD without any extra salt loading. The present study showed the potential of cut-soiler PSSD in root zone salinity management by improving drainage in salt-affected arid regions.

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    Sustainability
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    Authors: Ayushi Gaur; Abhishek Gaur; Dai Yamazaki; Slobodan P. Simonovic;

    This study discusses the flooding related consequences of climate change on most populous Canadian cities and flow regulation infrastructure (FRI). The discussion is based on the aggregated results of historical and projected future flooding frequencies and flood timing as generated by Canada-wide hydrodynamic modelling in a previous study. Impact assessment on 100 most populous Canadian cities indicate that future flooding frequencies in some of the most populous cities such as Toronto and Montreal can be expected to increase from 100 (250) years to 15 (22) years by the end of the 21st century making these cities highest at risk to projected changes in flooding frequencies as a consequence of climate change. Overall 40–60% of the analyzed cities are found to be associated with future increases in flooding frequencies and associated increases in flood hazard and flood risk. The flooding related impacts of climate change on 1072 FRIs located across Canada are assessed both in terms of projected changes in future flooding frequencies and changes in flood timings. Results suggest that 40–50% of the FRIs especially those located in southern Ontario, western coastal regions, and northern regions of Canada can be expected to experience future increases in flooding frequencies. FRIs located in many of these regions are also projected to experience future changes in flood timing underlining that operating rules for those FRIs may need to be reassessed to make them resilient to changing climate.

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    Water
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    Authors: Kenkichi Ishigure;

    Nuclear power generation is now one of the most important technologies in the energy supply in Japan. The operational experience of nuclear power plants for more than 20 years in Japan has shown that water chemistry is a key technology for safer and more economical operation of nuclear power plants. Extensive efforts have been made in the field of water chemistry to achieve the targets of radiation field control, to keep the integrities of piping and fuel elements and to reduce the radioactive waste generation. This paper briefly describes the present status of Japanese water chemistry technology with the main emphasis placed on BWRs.

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    Nuclear Engineering and Design
    Article . 1996 . Peer-reviewed
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      Nuclear Engineering and Design
      Article . 1996 . Peer-reviewed
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    Authors: Yoshitaka Sakata; Takao Katsura; Ahmed A. Serageldin; Katsunori Nagano; +1 Authors

    The variability of ground thermal conductivity, based on underground conditions, is often ignored during the design of ground-source heat pump systems. This study shows a field evidence of such site-scale variations through thermal response tests in eight borehole heat exchangers aligned at a site on a terrace along the foothills of mountains in northern Japan. Conventional analysis of the overall ground thermal conductivity along the total installation length finds that the value at one borehole heat exchanger is 2.5 times that at the other seven boreholes. History matching analysis of underground distributed temperature measurements generates vertical partial ground thermal conductivity data for four depth layers. Based on the moving line heat source theory, the partial values are generally within a narrow range expected for gravel deposits. Darcy velocities of groundwater are estimated to be 74–204 m/y at the borehole with high conductivity, increasing in the shallow layers above a depth of 41 m. In contrast, the velocities at the other seven boreholes are one-to-two orders of magnitude smaller with no trend. These high and low velocity values are considered for the topography and permeability. However, the relatively slow groundwater velocities might not apparently increase the partial conductivity.

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    Energies
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    Authors: M. Venkateswar Reddy; M. Venkateswar Reddy; Young-Cheol Chang;

    Medium-chain fatty acids (MCFA) such as caproic, heptanoic, and caprylic acids are monocarboxylic acids, which can be used as precursor molecules to synthesize biodiesel, bioplastics, antimicrobials, and corrosion inhibitors.

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    Sustainable Energy & Fuels
    Article . 2021 . Peer-reviewed
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      Sustainable Energy & Fuels
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    Authors: Ryota Koga; Miho Kitayama; Atsushi Kouzuma; Kazuya Watanabe; +1 Authors

    ABSTRACT An electrochemical flow cell equipped with a graphite working electrode (WE) at the bottom was inoculated with Shewanella oneidensis MR-1 expressing an anaerobic fluorescent protein, and biofilm formation on the WE was observed over time during current generation at WE potentials of +0.4 and 0 V (versus standard hydrogen electrodes), under electrolyte-flow conditions. Electrochemical analyses suggested the presence of unique electron-transfer mechanisms in the +0.4-V biofilm. Microscopic analyses revealed that, in contrast to aerobic biofilms, current-generating biofilm (at +0.4 V) was thin and flat (∼10 μm in thickness), and cells were evenly and densely distributed in the biofilm. In contrast, cells were unevenly distributed in biofilm formed at 0 V. In situ fluorescence staining and biofilm recovery experiments showed that the amounts of extracellular polysaccharides (EPSs) in the +0.4-V biofilm were much smaller than those in the aerobic and 0-V biofilms, suggesting that Shewanella cells suppress the production of EPSs at +0.4 V under flow conditions. We suggest that Shewanella cells perceive electrode potentials and modulate the structure and composition of biofilms to efficiently transfer electrons to electrodes. IMPORTANCE A promising application of microbial fuel cells (MFCs) is to save energy in wastewater treatment. Since current is generated in these MFCs by biofilm microbes under horizontal flows of wastewater, it is important to understand the mechanisms for biofilm formation and current generation under water-flow conditions. Although massive work has been done to analyze the molecular mechanisms for current generation by model exoelectrogenic bacteria, such as Shewanella oneidensis , limited information is available regarding the formation of current-generating biofilms over time under water-flow conditions. The present study developed electrochemical flow cells and used them to examine the electrochemical and structural features of current-generating biofilms under water-flow conditions. We show unique features of mature biofilms actively generating current, creating opportunities to search for as-yet-undiscovered current-generating mechanisms in Shewanella biofilms. Furthermore, information provided in the present study is useful for researchers attempting to develop anode architectures suitable for wastewater treatment MFCs.

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    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Applied and Environmental Microbiology
    Article . 2017 . Peer-reviewed
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Applied and Environm...arrow_drop_down
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      Applied and Environmental Microbiology
      Article . 2017 . Peer-reviewed
      License: ASM Journals Non-Commercial TDM
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      This Research product is the result of merged Research products in OpenAIRE.

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