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Lakes and rivers covered by seasonal ice are extensively used by humans. Although ice cover duration has been declining over the past 150 years for Northern Hemisphere lakes and rivers, we still know relatively little about how inland ice loss directly affects humans. Here we provide empirical examples that give quantitative evidence for a winter warming effect on a wide range of cultural ecosystem services. We show that in recent decades, warmer temperatures delayed the opening date of the James Bay winter ice road in northern Ontario, Canada and led to cancellations of religious celebrations (Lake Suwa, Japan and Lake Constance, Germany/Switzerland/Austria), an ice skating race on Lake M��laren, Sweden, and winter ice fishing tournaments in Central and Northern Minnesota. The years with no ice cover on Lake Suwa, Japan from 1443 - 2017. The years with ice cover on Lake Constance, Germany/Switzerland/Austria from 875 - 2018. The years with a normal Vikingar��nnet ice skating race, a modified route, or no race on Lake M��laren, Sweden from 1999 - 2017 as well as associated average winter air temperature. The years with canceled ice fishing tournaments in central and northern Minnesota, USA as well as associated average winter air temperature from 2005 - 2017. Road date openings of the James Bay winter ice road in northern Ontario, Canada and associated freezing degree days from 2005 - 2018.

Abstract Background The ascomycete fungus Podospora anserina has been appreciated for its targeted carbohydrate-active enzymatic arsenal. As a late colonizer of herbivorous dung, the fungus acts specifically on the more recalcitrant fraction of lignocellulose and this lignin-rich biotope might have resulted in the evolution of ligninolytic activities. However, the lignin-degrading abilities of the fungus have not been demonstrated by chemical analyses at the molecular level and are, thus far, solely based on genome and secretome predictions. To evaluate whether P. anserina might provide a novel source of lignin-active enzymes to tap into for potential biotechnological applications, we comprehensively mapped wheat straw lignin during fungal growth and characterized the fungal secretome. Results Quantitative 13C lignin internal standard py-GC–MS analysis showed substantial lignin removal during the 7 days of fungal growth (24% w/w), though carbohydrates were preferably targeted (58% w/w removal). Structural characterization of residual lignin by using py-GC–MS and HSQC NMR analyses demonstrated that Cα-oxidized substructures significantly increased through fungal action, while intact β-O-4′ aryl ether linkages, p-coumarate and ferulate moieties decreased, albeit to lesser extents than observed for the action of basidiomycetes. Proteomic analysis indicated that the presence of lignin induced considerable changes in the secretome of P. anserina. This was particularly reflected in a strong reduction of cellulases and galactomannanases, while H2O2-producing enzymes clearly increased. The latter enzymes, together with laccases, were likely involved in the observed ligninolysis. Conclusions For the first time, we provide unambiguous evidence for the ligninolytic activity of the ascomycete fungus P. anserina and expand the view on its enzymatic repertoire beyond carbohydrate degradation. Our results can be of significance for the development of biological lignin conversion technologies by contributing to the quest for novel lignin-active enzymes and organisms. Background The ascomycete fungus Podospora anserina has been appreciated for its targeted carbohydrate-active enzymatic arsenal. As a late colonizer of herbivorous dung, the fungus acts specifically on the more recalcitrant fraction of lignocellulose and this lignin-rich biotope might have resulted in the evolution of ligninolytic activities. However, the lignin-degrading abilities of the fungus have not been demonstrated by chemical analyses at the molecular level and are, thus far, solely based on genome and secretome predictions. To evaluate whether P. anserina might provide a novel source of lignin-active enzymes to tap into for potential biotechnological applications, we comprehensively mapped wheat straw lignin during fungal growth and characterized the fungal secretome. Results Quantitative 13C lignin internal standard py-GC–MS analysis showed substantial lignin removal during the 7 days of fungal growth (24% w/w), though carbohydrates were preferably targeted (58% w/w removal). Structural characterization of residual lignin by using py-GC–MS and HSQC NMR analyses demonstrated that Cα-oxidized substructures significantly increased through fungal action, while intact β-O-4′ aryl ether linkages, p-coumarate and ferulate moieties decreased, albeit to lesser extents than observed for the action of basidiomycetes. Proteomic analysis indicated that the presence of lignin induced considerable changes in the secretome of P. anserina. This was particularly reflected in a strong reduction of cellulases and galactomannanases, while H2O2-producing enzymes clearly increased. The latter enzymes, together with laccases, were likely involved in the observed ligninolysis. Conclusions For the first time, we provide unambiguous evidence for the ligninolytic activity of the ascomycete fungus P. anserina and expand the view on its enzymatic repertoire beyond carbohydrate degradation. Our results can be of significance for the development of biological lignin conversion technologies by contributing to the quest for novel lignin-active enzymes and organisms.

The Arctic is one of Earth’s regions most susceptible to climate change. However, the in-situ long-term observations used for climate research are relatively sparse in the Arctic Ocean, and the simulations from current climate models exhibit remarkable biases in the Arctic. Here we present an Arctic Ocean dynamical downscaling dataset based on a high-resolution ice-ocean coupled model FESOM and a climate model FIO-ESM. The dataset includes 115-year (1900–2014) historical simulations and two 86-year future scenario simulations (2015–2100) under scenarios SSP245 and SSP585. The historical results demonstrate that the root mean square errors of temperature and salinity in the dynamical downscaling dataset are much smaller than those from CMIP6 (the Coupled Model Intercomparison Project phase 6) climate models. The common biases, such as the too deep and too thick Atlantic layer in climate models, are reduced significantly by dynamical downscaling. This dataset serves as a crucial long-term data source for climate change assessments and scientific research in the Arctic Ocean, providing valuable information for the scientific community. The Arctic is one of Earth’s regions most susceptible to climate change. However, the in-situ long-term observations used for climate research are relatively sparse in the Arctic Ocean, and the simulations from current climate models exhibit remarkable biases in the Arctic. Here we present an Arctic Ocean dynamical downscaling dataset based on a high-resolution ice-ocean coupled model FESOM and a climate model FIO-ESM. The dataset includes 115-year (1900–2014) historical simulations and two 86-year future scenario simulations (2015–2100) under scenarios SSP245 and SSP585. The historical results demonstrate that the root mean square errors of temperature and salinity in the dynamical downscaling dataset are much smaller than those from CMIP6 (the Coupled Model Intercomparison Project phase 6) climate models. The common biases, such as the too deep and too thick Atlantic layer in climate models, are reduced significantly by dynamical downscaling. This dataset serves as a crucial long-term data source for climate change assessments and scientific research in the Arctic Ocean, providing valuable information for the scientific community.

A total of 170 participants were initially enrolled in the comprehensive behavioral weight loss intervention.In this study, investigators will conduct a follow-up visit 3 years after the completion of the intervention. Only participants who completed the behavioral weight loss intervention will be enrolled in this study. Participants will undergo testing of body weight, body composition, physical activity patterns, energy intake patterns, sleep patterns, resting metabolic rate, and total daily energy expenditure. This study is designed as an observational trial. The objective of this study is to follow-up with participants 3 years after completion of an 18-month comprehensive behavioral weight loss intervention. Outcomes of interest include change in body weight, body composition, physical activity, energy intake, and sleep. In addition, investigators will explore the associations between current physical activity, sleep, and energy intake patterns and body weight regulation.

# Data on: Projecting spatiotemporal dynamics of severe fever with thrombocytopenia syndrome in the mainland of China [https://doi.org/10.5061/dryad.vdncjsz1z](https://doi.org/10.5061/dryad.vdncjsz1z) This dataset is the data used in the paper of Global change biology entitled "Projecting spatiotemporal dynamics of severe fever with thrombocytopenia syndrome in the mainland of China". We use an integrated multi-model, multi-scenario framework to assess the impact of global climate change on SFTS disease in the mainland of China. ## Description of the data and file structure The predicted annual incidence of national SFTS cases with or without human population reduction under four RCPs under different climate change scenarios (RCP2.6, RCP4.5, RCP6.0, and RCP8.5) in the 2030s, 2050s, and 2080s. The value represents the annual incidence, and the unit is 105/year. The Dataset-1 file includes the predicted annual incidence of national SFTS cases with a fixed future human population under different climate change scenarios (RCP2.6, RCP4.5, RCP6.0, and RCP8.5) in the 2030s, 2050s, and 2080s. The Dataset-2 file includes the predicted annual incidence of national SFTS cases in the 2030s, 2050s, and 2080s with human population reduction (SSP2) under four RCPs. ## Sharing/Access information Data was derived from the following sources: * https://doi.org/10.1111/gcb.16969 This dataset is the data used in the paper of Global change biology entitled "Projecting spatiotemporal dynamics of severe fever with thrombocytopenia syndrome in the mainland of China". We use an integrated multi-model, multi-scenario framework to assess the impact of global climate change on SFTS disease in the mainland of China. The SFTS incidence in three time periods (2030-2039, 2050-2059, 2080-2089) is predicted to be increased as compared to the 2010s in the context of various RCPs. The projected spatiotemporal dynamics of SFTS will be heterogeneous across provinces. Notably, we predict possible outbreaks in Xinjiang and Yunnan in the future, where only sporadic cases have been reported previously. These findings highlight the need for population awareness of SFTS in endemic regions, and enhanced monitoring in potential risk areas.  See the Materials and methods section in the original paper. The code used in the statistical analyses are present in the paper and/or the Supplementary Materials.

Flight trajectories of fruit flies and mosquitoes in a wind tunnel.This data file is a MySQL database file which must be uploaded to a MySQL database management system (DBMS) (e.g., via the MAMP installation: http://localhost:8888/MAMP/?language=English, as was used in the associated manuscript). Once you have installed a MySQL DBMS on your machine, make a new database called “wind_tunnel_db”. To populate this database using the data file, first download all of the data files and join them together using: cat wind_tunnel_db_* > wind_tunnel_db.sql Then run the following command to populate the wind_tunnel_db MySQL database with the result. /path/to/mysql -uroot -proot wind_tunnel_db < /path/to/wind_tunnel_db.sql replacing the paths and username/passwords as appropriate. It will take several minutes since it is a large file. The database contains several tables, which are mostly self explanatory. The key tables of interest are the “experiment” table, which lists the 4 experiments contained in this data set, the “timepoint” table, which contains the position, velocity, etc., of every fly/mosquito at every measured time point, and the “trajectory” table, which indicates which set of time points correspond to which individual trajectories. Other useful tables that have been pre-populated are the “crossing” table, which specifies trajectory segments corresponding to each plume crossing, and the “crossing_group” table, which groups sets of crossings together according to experiment and crossing identification criteria. The code that interacts with this database and recreates the figures in the associated manuscript is contained at https://github.com/rkp8000/wind_tunnel.wind_tunnel_db_aaPart 2wind_tunnel_db_abPart 3wind_tunnel_db_acPart 4wind_tunnel_db_adPart 5wind_tunnel_db_aePart 6wind_tunnel_db_afPart 7wind_tunnel_db_agPart 8wind_tunnel_db_ahPart 9wind_tunnel_db_aiInfotaxis databaseBase database for running infotaxis simulations. To see how to prepare and populate this database with simulated trajectory data, see the file _paper_auxiliary_code in the GitHub repository http://github.com/rkp8000/wind_tunnel.infotaxis_db.sql Natural decision-making often involves extended decision sequences in response to variable stimuli with complex structure. As an example, many animals follow odor plumes to locate food sources or mates, but turbulence breaks up the advected odor signal into intermittent filaments and puffs. This scenario provides an opportunity to ask how animals use sparse, instantaneous, and stochastic signal encounters to generate goal-oriented behavioral sequences. Here we examined the trajectories of flying fruit flies (Drosophila melanogaster) and mosquitoes (Aedes aegypti) navigating in controlled plumes of attractive odorants. While it is known that mean odor-triggered flight responses are dominated by upwind turns, individual responses are highly variable. We asked whether deviations from mean responses depended on specific features of odor encounters, and found that odor-triggered turns were slightly but significantly modulated by two features of odor encounters. First, encounters with higher concentrations triggered stronger upwind turns. Second, encounters occurring later in a sequence triggered weaker upwind turns. To contextualize the latter history dependence theoretically, we examined trajectories simulated from three normative tracking strategies. We found that neither a purely reactive strategy nor a strategy in which the tracker learned the plume centerline over time captured the observed history dependence. In contrast, “infotaxis”, in which flight decisions maximized expected information gain about source location, exhibited a history dependence aligned in sign with the data, though much larger in magnitude. These findings suggest that while true plume tracking is dominated by a reactive odor response it might also involve a history-dependent modulation of responses consistent with the accumulation of information about a source over multi-encounter timescales. This suggests that short-term memory processes modulating decision sequences may play a role in natural plume tracking.

Lifestyle choices,including diet,are conducive to healthy body weights in children. Dairy products and calcium supplementation have been associated with moderation of body weight and body fat. This study was designed to test the following hypotheses with overweight and obese adolescents consuming a controlled diet: - Dietary calcium supplementation as calcium carbonate or dairy calcium modulates energy balance in adolescents. - Increased calcium in the diet of adolescents will increase fecal fat excretion and thereby decrease fat absorption. - Calcium and dairy product supplementation will increase lipid oxidation resulting in an increase in energy expenditure. Subjects will consume a controlled diet containing 800 mg calcium for two three week periods. During one period they will also receive a frozen ice cream like product (smoothie) twice a day based on soy protein that contains no additional calcium. During the other period they will receive a similar product twice a day based on either dairy protein that contains 650 mg calcium or based on soy protein that contains 650 mg calcium as calcium carbonate

Matlab program and data for the paper “An energy consumption rectification method based on Bayesian linear regression and heating degree-days". "simulation model.zip" is the heating house model in Trnsys simulation software. "Example1" and "Example2" is the Matlab program and data in this paper.