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Adaptation to a repeated restraint stress schedule was monitored in ethanol-treated and control rats. A single episode of 2 h restraint decreased food intake in both control and ethanol-treated rats. The decreases in control rats were not observed following the 5th daily restraint of 2 h/day, suggesting that adaptation has occurred. Ethanol-treated rats, however, exhibited decreased food intake even after 5th daily restraint of 2 h/day. Ethanol administration decreased weekly but not daily cumulative food intake in unrestrained rats. Food intakes of ethanol-treated and control restrained rats were comparable following 1st-3rd daily restraints, but were smaller in ethanol-treated rats following the 4th and 5th daily restraints. Open-field ambulatory activities monitored 24 h after the 5th daily restraint on the 6th day were comparable in control restrained and unrestrained rats. Ethanol-treated and control unrestrained rats also exhibited comparable ambulation, but ethanol-treated rats exhibited smaller activity than control restrained or ethanol-treated unrestrained rats. Fluid intakes of ethanol and control rats were comparable during the 2 weeks of ethanol administration, but daily restraint schedule decreased ethanol intake. The findings show adaptation to repeated restraint in control rats and inability of ethanol-treated rats to adapt in the stress schedule. These findings imply that excessive alcohol consumption may impair adaptation to stress and thus conceivably precipitate depression.

The energy crisis and increasing fossil fuel prices due to increasing demands, controlled supplies, and global political unrest have adversely affected agricultural productivity and farm profitability across the globe and Pakistan is not an exception. To cope with this issue of energy deficiency in agriculture, the best alternate strategy is to take advantage of biomass and solid waste potential. In low-income countries such as Pakistan, the greenhouse heating system mostly relies on fossil fuels such as diesel, gasoline, and LPG. Farmers are reluctant to adopt greenhouse farming due to the continuously rising prices of the fossil fuels. To reduce reliance on fossil fuel energy, the objective of this study was to utilize biomass from crop residues to develop an efficient and economical biomass furnace that could heat greenhouses to protect the crop from seasonal temperature effects. Modifications made to the biomass furnace, such as the incorporation of insulation around the walls of the furnace, providing turbulators in fire tubes, and a secondary heat exchanger (heat recovery system) in the chimney, have increased the thermal efficiency of the biomass furnace by about 21.7%. A drastic reduction in hazardous elements of flue gases was observed due to the addition of a water scrubber smoke filter in the exit line of the flue. The efficiency of the biomass furnace ranged from 50.42% to 54.18%, whereas the heating efficiency of the diesel-fired heater was 71.19%. On the basis of the equal heating value of the fuels, the unit material and operating costs of the biomass furnace for wood, cotton stalks, corn cobs, and cow dung were USD 2.04, 1.86, 1.78, and 2.00 respectively against USD 4.67/h for the diesel heater. The capital and operating costs of the biomass furnace were about 50% and 43.7% of the diesel heater respectively, resulting in a seasonal saving of about 1573 USD. The produced smoke was tested as environmental friendly under the prescribed limits of the National Environmental Quality Standards (NEQS), which shows potential for its large-scale adoption and wider applications.

Biogas production is a clean renewable energy source that can improve lives in developing countries. However, winter temperatures in some areas are too low to enable enough biogas production in small unheated digesters to meet the energy requirements of households. Low-cost, high yield reactors adapted to the local climate are needed in those situations. A decision-support model was developed to assist in the design of biogas reactors capable of meeting households’ year-round energy needs. Monthly biogas production relative to household energy needs was calculated for the scenario of suburban Hanoi, Vietnam. Calculations included pig number, slurry (manure water mixture) dilution, retention time and biogas/solar heating. Although using biogas to heat the digester increased biogas production, it did not lead to an energy surplus, particularly with the 1:9 slurry dilution rate commonly used on pig farms. However, at a 1:3 slurry dilution, the use of solar heating to provide 90% and biogas 10% of the heat required to heat the digester to 35 °C improved the biogas production by 50% compared to psychrophilic production. The energy needs of an average five-person family throughout the year required 17 fattening pigs. This model can establish the best solution for producing sufficient energy throughout the year.

It is increasingly acknowledged that climate change is influencing terrestrial ecosystems by increased drought and rainfall intensities. Soil microbes are key drivers of many processes in terrestrial systems and rely on water in soil pores to fulfill their life cycles and functions. However, little is known on how drought and rainfall fluctuations, which affect the composition and structure of microbial communities, persist once original moisture conditions have been restored. Here, we study how simulated short-term drying and re-wetting events shape the community composition of soil fungi and prokaryotes. In a mesocosm experiment, soil was exposed to an extreme drought, then re-wetted to optimal moisture (50% WHC, water holding capacity) or to saturation level (100% WHC). Composition, community structure and diversity of microbes were measured by sequencing ITS and 16S rRNA gene amplicons 3 weeks after original moisture content had been restored. Drying and extreme re-wetting decreased richness of microbial communities, but not evenness. Abundance changes were observed in only 8% of prokaryote OTUs, and 25% of fungal OTUs, whereas all other OTUs did not differ between drying and re-wetting treatments. Two specific legacy response groups (LRGs) were observed for both prokaryotes and fungi. OTUs belonging to the first LRG decreased in relative abundance in soil with a history of drought, whereas OTUs that increased in soil with a history of drought formed a second LRG. These microbial responses were spread among different phyla. Drought appeared to be more important for the microbial community composition than the following extreme re-wetting. 16S profiles were correlated with both inorganic N concentration and basal respiration and ITS profiles correlated with fungal biomass. We conclude that a drying and/or an extreme re-wetting history can persist in soil microbial communities via specific response groups composed of members with broad phylogenetic origins, with possible functional consequences on soil processes and plant species. As a large fraction of OTUs responding to drying and re-wetting belonged to the rare biosphere, our results suggest that low abundant microbial species are potentially important for ecosystem responses to extreme weather events.

Sustainable food supply has gained considerable consumer concern due to the high percentage of spoilage microorganisms. Food industries need to expand advanced technologies that can maintain the nutritive content of foods, enhance the bio-availability of bioactive compounds, provide environmental and economic sustainability, and fulfill consumers’ requirements of sensory characteristics. Heat treatment negatively affects food samples’ nutritional and sensory properties as bioactives are sensitive to high-temperature processing. The need arises for non-thermal processes to reduce food losses, and sustainable developments in preservation, nutritional security, and food safety are crucial parameters for the upcoming era. Non-thermal processes have been successfully approved because they increase food quality, reduce water utilization, decrease emissions, improve energy efficiency, assure clean labeling, and utilize by-products from waste food. These processes include pulsed electric field (PEF), sonication, high-pressure processing (HPP), cold plasma, and pulsed light. This review describes the use of HPP in various processes for sustainable food processing. The influence of this technique on microbial, physicochemical, and nutritional properties of foods for sustainable food supply is discussed. This approach also emphasizes the limitations of this emerging technique. HPP has been successfully analyzed to meet the global requirements. A limited global food source must have a balanced approach to the raw content, water, energy, and nutrient content. HPP showed positive results in reducing microbial spoilage and, at the same time, retains the nutritional value. HPP technology meets the essential requirements for sustainable and clean labeled food production. It requires limited resources to produce nutritionally suitable foods for consumers’ health.

Differences in bacterial community composition (BCC) between bulk and rhizosphere soil and between rhizospheres of different plant species are assumed to be strongly governed by quantitative and qualitative rhizodeposit differences. However, data on the relationship between rhizodeposit amounts and BCC are lacking. Other soil microorganisms, e.g. arbuscular mycorrhizal fungi (AMF), may also influence BCC. We simulated foliar herbivory (cutting) to reduce belowground carbon allocation and rhizodeposition of pea plants grown either with or without AMF. This reduced soil respiration, rhizosphere microbial biomass and bacteriovorous protozoan abundance, whereas none of these were affected by AMF. After labelling plants with (13)CO(2), root and rhizosphere soil (13)C enrichment of cut plants were reduced to a higher extent (24-46%) than shoot (13)C enrichment (10-24%). AMF did not affect (13)C enrichment. Despite these clear indications of reduced rhizosphere carbon-input, denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes PCR-amplified targeting DNA and RNA from rhizosphere soil did not reveal any effects of cutting on banding patterns. In contrast, AMF induced consistent differences in both DNA- and RNA-based DGGE profiles. These results show that a reduction in rhizosphere microbial activity is not necessarily accompanied by changes in BCC, whereas AMF presence inhibits proliferation of some bacterial taxa while stimulating others.

Agriculture is the largest sector of Pakistan’s economy, contributing almost 22% to the GDP and employing almost 45% of the total labor force. The two largest food crops, wheat and rice, contribute 3.1% and 1.4% to the GDP, respectively. The objective of this research was to calculate the energy return on investment (EROI) of these crops on a national scale from 1999 to 2009 to understand the size of various energy inputs and to discuss their contributions to the energy output. Energy inputs accounted for within the cropping systems included seed, fertilizer, pesticide, human labor, tractor diesel, irrigation pump electricity and diesel, the transport of fertilizer and pesticide, and the embodied energy of tractors and irrigation pumps. The largest per-hectare energy inputs to wheat were nitrogen fertilizer (52.6%), seed (17.9%), and tractor diesel (9.1%). For rice, the largest per-hectare energy inputs were nitrogen fertilizer (32%), tube well diesel (19.8%), and pesticide (17.6%). The EROI of wheat showed a gradual downward trend between 2000 and 2006 of 21.3%. The trend was erratic thereafter. Overall, it ranged from 2.7 to 3.4 with an average of 2.9 over the 11-year study period. The overall trend was fairly consistent compared to that of rice which ranged between 3.1 and 4.9, and averaged 3.9. Rice’s EROI dipped sharply in 2002, was erratic, and remained below four until 2007. It rose sharply after that. As energy inputs increased, wheat outputs increased, but rice outputs decreased slightly. Rice responded to inputs with greater output and an increase in EROI. The same was not true for wheat, which showed little change in EROI in the face of increasing inputs. This suggests that additional investments of energy in rice production are not improving yields but for wheat, these investments are still generating benefits. The analysis shows quantitatively how fossil energy is a key driver of the Pakistani agricultural system as it traces direct and indirect energy inputs to two major food crops.