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Seabass (Lates calcarifer) is one of the top farmed and raised fish in Taiwan, and fish scales are the main by-product after processing. Fish scales contain high amounts of collagen, which can chelate with minerals and enhance mineral absorption in the human body. Hence, fish scales from seabass were enzymatically hydrolyzed to obtain seabass scale collagen peptide (SBSCP). Calcium, the most consumed mineral supplement, was chelated with SBSCP to form SBSCP-Ca. The optimal conditions for chelation were a peptide/calcium ratio: 1:1 (w/w), pH 5.0, and 50 °C for 20 min. The conjugated sites were carboxyl and amino groups based on Fourier transform infrared (FTIR) spectroscopy. Scanning electronic microscope/energy-dispersive X-ray spectroscopy (SEM/EDS) evidently showed the alternation of SBSCP’s molecular structure after chelation and increased concentrations of metal ions. SBSCP-Ca was stable up to 90 °C and from pH 2.0 to 5.0. The retention rate was 70%, as determined after in vitro digestion. The extracts of blackcurrant or berry-grape seeds were added to neutralize the fishy odor and provide antioxidant ability for commercialization. This is the first complete study of the characteristics of SBSCP-Ca as well as their commercialization.

Dark fermentation (DF) is a simple method for hydrogen (H2) production through the valorization of various organic wastes that can be used as feedstock. In particular, an organic fraction of municipal solid waste (OFMSW) is a fermentation substrate that can easily be gathered and provides high yields in biogas and value-added organic compounds such as volatile fatty acids (VFAs). DF is coupled with a methanogenic reactor to enhance biogas production from the OFMSW. In this study, a two-stage reactor was conducted and monitored to optimize the methane yield by reducing the HRT at the DF reactor. A focus of the functional inference based on a next-generation sequence (NGS) metabarcoding analysis and comparison of microbial communities that populate each reactor stage was performed. Concerning gas quality, the two-stage system observed a hydrogen-rich biogas in the first fermentative reactor (on average 20.2%) and an improvement in the methane content in the second methanogenic digester, which shifted from 61.2% obtained for the one-stage experiment to 73.5%. Such increases were due to the improvement in substrate hydrolysis. As for the specific biogas production, the results showed an overall increase of 50%.

This paper aims to identify the causes and sources of erosion and deposition at small estuaries in southern central Vietnam under human intervention. The jetty built at the Tam Quan river mouth (Binh Dinh Province, Vietnam) serves as the base for the study. After its completion at the end of 2009, the hydrodynamic and erosion-deposition processes in the region have been significantly altered. Inside the estuary, the waves are not influenced, but the currents are increased during the ebb tide period and decreased during the flood tide timeframe. During the southwest monsoon, the jetty could cause an increase in the deposition process in both frequency and area, whereas the erosion process tends to narrow the area and increase the frequency on the north coast. In contrast, both deposition and erosion processes are increased on the southern coast. About 5859 m3 of sediments are deposited in the channel gate mainly by local sources. During the northeast monsoon, both deposition and erosion processes are located over a narrow area with frequency increased on the north coast, whereas the deposition process is narrowed with higher frequency on the southern coast. The total amount of sediment deposited at the estuary is 56,446 m3, of which 74.2% is from the onsite erosion material, 15.8% from the river and 10% from the longshore transportation. Generally, due to mainly erosion-deposition processes, sediment volume is accumulated during the northeast monsoon with amount 9.6 times more than that the southwest monsoon. The erosion-deposition processes are contributed to by poor practical management and local human activities inland and in the coastal regions, as well as the natural situation, resulting in serious impacts on society, the economy and the environment. Hence, the governance of the erosion-deposition processes and sediment load in small estuaries appear to contribute to the master plan for the local sustainable development of society and the economy.

This study evaluated the efficacy of enzyme induced calcite precipitation (EICP) in restricting the mobility of heavy metals in soils. EICP is an environmentally friendly method that has wide ranging applications in the sustainable development of civil infrastructure. The study examined the desorption of three heavy metals from treated and untreated soils using ethylene diamine tetra-acetic acid (EDTA) and citric acid (C6H8O7) extractants under harsh conditions. Two natural soils spiked with cadmium (Cd), nickel (Ni), and lead (Pb) were studied in this research. The soils were treated with three types of enzyme solutions (ESs) to achieve EICP. A combination of urea of one molarity (M), 0.67 M calcium chloride, and urease enzyme (3 g/L) was mixed in deionized (DI) water to prepare enzyme solution 1 (ES1); non-fat milk powder (4 g/L) was added to ES1 to prepare enzyme solution 2 (ES2); and 0.37 M urea, 0.25 M calcium chloride, 0.85 g/L urease enzyme, and 4 g/L non-fat milk powder were mixed in DI water to prepare enzyme solution 3 (ES3). Ni, Cd, and Pb were added with load ratios of 50 and 100 mg/kg to both untreated and treated soils to study the effect of EICP on desorption rates of the heavy metals from soil. Desorption studies were performed after a curing period of 40 days. The curing period started after the soil samples were spiked with heavy metals. Soils treated with ESs were spiked with heavy metals after a curing period of 21 days and then further cured for 40 days. The amount of CaCO3 precipitated in the soil by the ESs was quantified using a gravimetric acid digestion test, which related the desorption of heavy metals to the amount of precipitated CaCO3. The order of desorption was as follows: Cd > Ni > Pb. It was observed that the average maximum removal efficiency of the untreated soil samples (irrespective of the load ratio and contaminants) was approximately 48% when extracted by EDTA and 46% when extracted by citric acid. The soil samples treated with ES2 exhibited average maximum removal efficiencies of 19% and 10% when extracted by EDTA and citric acid, respectively. It was observed that ES2 precipitated a maximum amount of calcium carbonate (CaCO3) when compared to ES1 and ES3 and retained the maximum amount of heavy metals in the soil by forming a CaCO3 shield on the heavy metals, thus decreasing their mobility. An approximate improvement of 30% in the retention of heavy metal ions was observed in soils treated with ESs when compared to untreated soil samples. Therefore, the study suggests that ESs can be an effective alternative in the remediation of soils contaminated with heavy metal ions.

Water scarcity is a driver for society to rethink water management and change the paradigm of use to a fit-for-purpose approach—i.e., separating potable water for human consumption (drinking, cooking or personal hygiene) from all non-potable uses that do not require the same quality level. In this context, urban water reuse is a relevant tool for municipalities and metropolitan areas when dealing with pressure on water resources, among several alternative water sources that can be considered in a site-specific and integrated manner. Through the available literature and specific case studies in the Lisbon Metropolitan Area, this paper explores the benefits and barriers of water reuse and intends to support local authorities in including water reuse in their water management strategies. To the best of our knowledge, this is the first paper focusing on Portugal and the Lisbon Metropolitan Area that globally examines governance, economic, legislative and social aspects regarding water reuse and presents specific implementation examples covering potable and non-potable as well as direct and indirect reuse.

Bioenergy is expected to play a key role in achieving a future sustainable energy system. Sweet sorghum-based fuel ethanol, one of the most promising bioenergy sources in China, has been receiving considerable attention. However, the conflict between sweet sorghum development and traditional water use has not been fully considered. The article presents an integrated method for evaluating water stress from sweet sorghum-based fuel ethanol in China. The region for developing sweet sorghum was identified from the perspective of sustainable development of water resources. First, the spatial distribution of the water demand of sweet sorghum-based fuel ethanol was generated with a Decision Support System for Agrotechnology Transfer (DSSAT) model coupled with Geo-Information System (GIS). Subsequently, the surplus of water resources at the provincial scale and precipitation at the pixel scale were considered during the growth period of sweet sorghum, and the potential conflicts between the supply and demand of water resources were analyzed at regional scale monthly. Finally, the development level of sweet sorghum-based fuel ethanol was determined. The results showed that if the pressure of water consumption of sweet sorghum on regional water resources was taken into account, about 23% of the original marginal land was not suitable for development of sweet sorghum-based fuel ethanol, mainly distributed in Beijing, Hebei, Ningxia, Shandong, Shanxi, Shaanxi, and Tianjin. In future energy planning, the water demand of energy plants must be fully considered to ensure its sustainable development.

An original methodology for the Water Footprint Assessment (WFA) of a Product for the wine-making industry sector is presented, with a particular focus on the evaluation procedure of the grey water. Results obtained with the proposed methodology are also presented for an Italian case study. The product was analyzed using a life-cycle approach, with the aim of studying the water volumes of each phase according to the newly-released ISO 14046 international standard. The functional unit chosen in this study is the common 0.75 liter wine bottle. An in-house software (V.I.V.A.) was implemented with the goal of accounting for all the contributions in a cradle-to-grave approach. At this stage, however, minor water volumes associated with some foreground and background processes are not assessed. The evaluation procedure was applied to a case study and green, blue, and grey water volumes were computed. Primary data were collected for a red wine produced by an Umbrian wine-making company. Results are in accordance with global average water footprint values from literature, showing a total WF of 632.2 L/bottle, with the major contribution (98.3%) given by green water, and minor contributions (1.2% and 0.5%) given by grey and blue water, respectively. A particular effort was dedicated to the definition of an improved methodology for the assessment of the virtual water volume required to dilute the load of pollutants on the environment below some reference level (Grey WF). The improved methodology was elaborated to assure the completeness of the water footprint assessment and to overcome some limitations of the reference approach. As a result, the overall WF can increase up to 3% in the most conservative hypotheses.

Castors are used to remediate heavy-metal-polluted soils due to their good ability to accumulate heavy metals. However, only limited studies addressed the interaction between heavy metals and castor seedling roots. In this study, the physiological response of castor seedling roots to Cd and Zn stress, and the change in trace elements in rhizosphere and non-rhizosphere soils were investigated. The results showed that, with an increase in Cd concentration, the accumulation of Zn in roots decreased by 20%, indicating a competition between Cd and Zn accumulation. The increase in Cd content enlarged the difference in nutrient contents at different depths: the amounts of P, Fe, and Mn were more in rhizosphere soils than in non-rhizosphere soils, while the amount of K showed an opposite trend. The addition of Cd and Zn stimulated root growth, but root activity was reduced. The addition of Cd and Zn affected the root cell morphology, including increases in the root cortex thickness and the root xylem area. The contents of the enzymes SOD, POD, and MDA increased with the addition of Cd and Zn, while the activity of CAT first increased and then decreased. There was no significant change in the soluble protein content. The decrease in IAA oxidase content, from 40.1% to 72.7%, was conducive to plant growth. To sum up, high contents of Cd and Zn in soils not only affect the root morphology and increase the gap in the contents of K, P, Fe, and Mn between rhizosphere and non-rhizosphere soils but also change the SOD, POD, MDA, and IAA contents in the root, so as to reduce the amount of root damage caused by the external environment.