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Cells respond to environmental cues by modifying protein complexes in the nucleus to produce a change in the pattern of gene expression. In this article, we review techniques that allow us to visualize these protein interactions as they occur in living cells. The cloning of genes from marine organisms that encode fluorescent proteins provides a way to tag and monitor the intracellular behavior of expressed fusion proteins. The genetic engineering of jellyfish green fluorescent protein (GFP) and the recent cloning of a sea anemone red fluorescent protein (RFP) have provided fluorescent tags that emit light at wavelengths ranging from the blue to the red spectrum. Several of these color variants can be readily distinguished by fluorescence microscopy, allowing them to be used in combination to monitor the behavior of two or more independent proteins in the same living cell. We describe the use of this approach to examine where transcription factors are assembled in the nucleus. To demonstrate that these labeled nuclear proteins are interacting, however, requires spatial resolution that exceeds the optical limit of the light microscope. This degree of spatial resolution can be achieved with the conventional light microscope using the technique of fluorescence resonance energy transfer (FRET). The application of FRET microscopy to detect the interactions between proteins labeled with the color variants of GFP and the limitations of the FRET approach are discussed. The use of different-color fluorescent proteins in combination with FRET offers the opportunity to study the complex behavior of key regulatory proteins in their natural environment within the living cell.

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The local implications of the well-known flux conservation equations of Morton et al. (Proc. R. Soc. Lond. A, vol. 234, 1956, p.1) for plumes and jets are considered. Given the vertical velocity distributions of a model plume or jet, the divergence-free radial velocity distributions are calculated. It is shown that in general the velocity of the plume boundary is not described by the local total fluid velocity in this way. A two-fluid model tracking the evolution of both ‘plume’ and ‘ambient’ fluid is proposed which resolves this apparent inconsistency and also provides a way of explicitly describing the mixing process within a model plume. The plume boundary acts as a phase boundary across which ambient fluid is entrained, and the plume boundary moves at the velocity of the plume fluid. The difference between the plume-fluid radial velocity and the total fluid velocity quantifies in a natural way the purely horizontal entrainment flux of ambient fluid into the plume across the phase boundary at the plume edge.

Territorial cichlids were presented for 30 min with a conspecific male intruder (contained in a clear glass tube). Eight hr prior, 2 groups were administered alcohol (0.15 or 0.30 percent in the aquaria water). A third group served as a control. Three responses were recorded to allow analysis of topographic changes in behavior as well as changes in absolute levels. During the habituation phase, the normal group showed a sequence of long displays, followed by shorter ones as the frequency of attacks increased. The occurrence of threat which gradually gives way to attack is characteristic of the agonistic behavior of this species. In contrast to the controls, the 0.15 percent group was hyperaggressive, while the 0.30 percent group was hypoaggressive. Furthermore, the patterning of responses were abnormal. The 0.15 percent group gave abbreviated threats and more attacks (interpreted as a tendency to attack without warning); whereas, the 0.30 percent group gave many long threat displays, but few attacks. A stimulus specificity test provided strong evidence that the waning found during the initial phase was habituation.

AbstractW and WSix films have been etched in Cl2 and Cl2/BCl3 plasmas using two different reactor designs (radial and flow systems, 0.1‐1.0 torr Cl2, 30‐170 °C).

This analysis employs the most recent scientific data for the U.S. and for Brazil sugarcane production and the fermentation/distillation. These two countries were selected because they are the two largest countries in the world producing ethanol. All current fossil energy inputs used in the entire process of producing ethanol from sugarcane were included to determine the entire energy cost for ethanol production. Additional costs to consumers, including federal and state subsidies, plus costs of environmental pollution and/or degradation associated with the entire production system are discussed. The economic and the broad human food supply issues are evaluated. In addition, other studies are compared.

The addition of ethanol is an extra step in preparation and may cause toxicity. For these reaseons, we studied the solubility and stability of carmustine in 5% dextrose/water