The EU28 imports 77% of the protein it requires, for food or feed, representing an important vulnerability for our economy, risking our self-sufficiency and food security. GreenProtein aims at a major innovation by producing high-added value, food-grade and fully functional proteins and other ingredients, out of vegetal residues mainly from sugar beet. The main outcome will be the valorisation of 4,000 tons of agricultural residue /yearly into a high quality dried RuBisCo powder ready to enter into food industry production chains as an alternative of the widely extended egg white preparations or whey protein. We are the first team worldwide, covering the whole food chain, to design an extraction system of functional RuBisCO. The project will design and implement specific equipment to establish a demonstration plant with capacity of 1,500 kg/hour of vegetal residue input, rendering 28 Kg of dry RuBisCO and 82 Kg of dry dietary fibre (25-30% dry matter). The obtained protein has top valuable functionalities required in food industry, such as gelling, foaming or emulsifying. The outcomes have excellent market projection due to their application in growing consumer needs like high-protein foodstuff, vegan, halal, etc. Establishing this DEMO plant will allow fine-tuning the extraction process at industrial scale in order to define the optimal conditions for industrial manufacture. It will be built in a compact design, ready to be “plugged” to the production line, an easily replicable system with a high spreading projection in whole EU.

Mixed bio-waste generated along the agri-food value chain is an abundant resource, rich in valuable compounds e.g., polysaccharides or polyphenolic constituents. Yet, its potential as a source of recycled components is untapped - 75% of the generated bio-waste (approx. 85 M/y) is landfilled or incinerated, constituting 3% of total EU GHG emissions. Valorisation of mixed bio-waste from the agri-food sector is hindered by (1) technological challenges of removing impurities (e.g., plastics, cardboard, or metals) that would otherwise decrease the process quality, (2) logistics challenges of collecting seasonal, geographically dispersed feedstock that lower valorisation cost-benefit. MixMatters proposes an innovative system that efficiently separates and valorises three types of mixed bio-waste streams containing impurities from the agri-food industry (wholesale markets, greenhouses, food and drink industry) and obtains six high value-added outputs (powder ingredients, sugar concentrates, recombinant proteins, green fibres, bioactive compounds, plastic monomers). The MixMatters system is modular and multi-purpose - able to treat a range of mixed bio-waste streams (ensuring feedstock supply). The separation stage is automated, integrating advanced robotics and AI. It is also containerised separating the bio-waste at waste generation sites to avoid inefficient transportation of water and stream spoilage. The system functioning is optimised via a Decision Support while transparency + traceability are ensured via a Digital Product Passport. In the project, the system will be demonstrated during 15 months at three waste generation sites, along with output validation with 6 bio-based industries and active involvement of value chain actors (partner networks outreach + 2,280 members) to ensure result deployment. MixMatters will contribute to meeting the separation targets of the Waste Framework Directive (separated 6,240 t/y by one system at industrial scale).