The increases of population lifetime and of the accidents are the two main reasons explaining the growing interest of the scientific community in studying the osteoarticular system. Although implant and osteoarticular prostheses have been widely used in clinical routine since more than 30 year and have allowed considerable therapeutic and esthetic improvements, a lot of optimizations and developments of their performances remain to be done. In particular, dental implants are widely used for maxillofacial rehabilitation purposes, with more than 400 000 implant surgery per year in France. Many cases of failure still happen due to a bad timing in the implant loading with the prosthesis. This is due to the fact that a reliable tool capable of verifying the quality of osseointegration is still missing. Such failures induce pain, degraded mastication conditions for patients and increased costs for dental surgeons. It still remains difficult to assess the stability of a dental implant and in particular the biomechanical properties of newly formed bone tissue around the implant. OsseoWave aims at developing an evaluation tool to assess the implant stability and to follow the implant osseointegration in the osteoarticular system. The first application to be transferred will be dental implants. The MSME laboratory of University Paris-Est has developed a new method for the follow-up of implants, which is sensitive to the bone-implant interface quality, the only accurate criteria for the implant surgical success. The system uses quantitative ultrasound analysis, which is a non invasive, non radiating and relatively cheap approach. A proof of concept has been demonstrated ex vivo and in vivo, which has allowed a French and PCT patent application and a new statement of invention. We intend to keep on working on in vivo validation experiments in rabbits and to start new ones in dogs in conditions closer to the clinical situation. Moreover, the development of numerical simulation tools will lead to the optimization of the signal processing methods used in the software of the device, which will improve the overall performances of the device. An industrialization study aim at conceiving and manufacturing the final version of the device. Then, the device will be validated in the framework of a preliminary clinical study and the results will be protected through new patent applications. The present project will bring the technology to the CE certification, which is necessary in order to carry out a clinical investigation at a larger scale in the framework of a PHRC funding. The present project will pave the way towards for other applications (ankle, hip and spine among others). The team is constituted by members with complementary skills (dental surgeons experts in oral biology of bone remodeling and researchers specialized in quantitative ultrasound imaging). The project has won the concours national de Création d’entreprise in Emergence and was founded through an Aima project by the Centre Francilien pour l’Innovation and through the ANR project WaveImplant (end: September 2013). These funding have been used to realize an intellectual property study which has shown the freedom to operate of the device and a first in vivo validation. Moreover, the laboratory has been contacted by two leading companies of the dental field (Septodont and Zimmer Dental) for the realization of a dedicated study. The developed technology has been under industrial transfer since mid-2009 which will be concretized through a grant of patent and know-how license to a company to be created and already incubated. The developed technology has the potential to justify a start-up creation because of: - An important, international and growing market with no effective competitors - Multiple possible applications of the technology - Need for a technical expertise but also for a strong industrial and marketing environment

The goal of BIO ART is to develop new bio-epoxy resins from renewable resources without bisphenol A, which is toxic to humans and the environment. BIO ART’s originality comes from the synergy between green chemistry and emerging technologies (multiscale modeling and artificial neural network) and sustainable application in industry. In contrast to purely experimental or exclusively numerical approaches, BIO ART integrates simulations and experiments at length and time scales ranging from the atomistic level to the engineering scale. The proposed project will contribute to close the four knowledge gaps: i) use of exclusively bio-sourced molecules from abundant resources and natural fillers with competitive mechanical properties, ii) multiscale modeling of epoxy including its macromolecular network topology, iii) optimization of the resin formulation by an artificial neural network framework linking the chemical nature of the molecules to the mechanical properties, and iv) advanced mechanical characterization and processing of fiber-reinforced bio-composites. BIO ART’s consortium consists of four complementary Franco-German partners with recognized skills in the synthesis of bio-polymers and physicochemical characterization (ICMPE/FR), in microstructure generation and surrogate models based on artificial neural networks (MSME/FR) as well as in multiscale modeling of polymers and discrete-to-continuum coupling methods (FAU/DE), and in composite processing and advanced mechanical characterization (UBT/DE). The scientific program is divided into 5 work packages: WP1: Synthesis of bio-sourced epoxy, WP2: Characterization of bio-sourced epoxy, WP3: Multiscale modeling, WP4: Optimization of bio-sourced epoxy formulation by artificial neural network, and WP5: Composite processing and mechanical characterization. The work packages are defined in a way that they can be completed in 3 years by 3 collaborating doctoral researchers, one for experimental part and two for the numerical part. A technician will support the experimental PhD candidate as regards the processing and characterization of the obtained materials. Beyond them, BIO ART’s consortium, which is a well-balanced composition of early career and senior scientists, will actively contribute to achieve the project’s milestones. BIO ART’s methods are up-to-date, are based on recently published results, and benefit from the strong synergies with current projects of the project partners. In particular, the experimental and numerical methods will range from the atomistic scale (molecular structure, synthesis of constituents, molecular dynamics simulations), to the mesoscale (curing process, network characterization, network model), and to the macroscale (fracture properties, continuum mechanical simulations). This methodology will focus on the investigation of the relationship between the structure and the multiscale properties of the obtained materials. This approach will synergistically combine modelling with experimental characterizations, which will allow to address the scientific issues of this project. This multidisciplinary scientific approach will allow BIO ART to respond to a current crucial societal issue, i.e. biosourced polymer materials from circular bio-economy, aimed for sustainable development applications

Vision and other light-based signaling processes are ubiquitous in Nature. Among all the molecular mechanisms responsible for light emission (eg fluorescence, phosphorescence …), chemiluminescence and its corresponding process in living organisms, bioluminescence, are interesting because of their molecular origin: chemical energy converted to light. It is noteworthy that the emitted light is a signature of the underlying chemical reaction. Hence bioluminescence has inspired the development of many analytical toolboxes in biomedical applications (eg imaging) using analogues of typical light emitters (fireflies, beetles, jellyfishes, ...) Because these analogues exhibit photochemical properties similar to their natural counterparts (luminophores), they usually emit blue and green colors, i.e. synthetic yellow or red light emitters remain scarce. The design of bio-inspired light emitters requires a deeper understanding of the factors responsible at the molecular level for the tuning of the emitted color. As exemplified by the design of Green Fluorescent Protein (GFP, 2008 Nobel Prize in Chemistry)-like systems with optimum photophysical properties, it is of paramount importance to deeply understand the interactions between the light-active species and their protein binding pocket. Similar achievement is needed for the development of bioluminescence-based highly sensitive analytical techniques in environmental, medical, food analysis to cite just a few. The BIOLUM project aims to combine state-of-the-art theoretical and experimental fundamental researches to assess the effect of two major color tuning factors: 1) the luminophore + protein structures and interactions, 2) pH. Crossing the informations accumulated for both native and modified luciferin luminophores in the paradigmatic firefly luciferase enzyme, we envision an improved picture of the mechanisms at work in color modulation, with focus on 1) structures of native and modified luciferins in the luciferase binding pocket, 2) relevant photochemical steps in light emission, 3) key proton transfers between oxyluciferin (denoting the very last luciferin structure before light emission) and various donors and acceptors and 4) amino acids of the protein binding pocket that are crucial to control light emission color. Finally, the gained knowledge will help to suggest new directions for the rational design of man-taylored chemi- and bio-luminescent systems with selected colors.

The subject of this project is the 3D printing (SLS) of PA12/glass beads composite for applications in aerospace industry. The SLS process uses laser sintering of composite powder with polymer matrix containing glass beads. One of the limiting points of polymers composites for their use in aerospace systems is their durability, and more specifically their resistance to failure due to fatigue cracking. The objective of this project will focus on the study of finished products obtained by SLS of composites powders and their resistance to cracking. The objectives of this work are to understand failure mechanisms in these highly heterogeneous materials at two scales, the scale of the microsctructure and the scale of the workpiece, by combining experimental characterization of cracks networks by mechanical testing, 3D imaging by X-rays laboratory microtomography image analysis, and numerical simulations. The identified microstructural damage models will be used to construct a crack propagation model at the scale of the workpieces, and will account for specificities related to the material and the process: the highly heterogeneous nature of the microstructure and its strong anisotropy due to the layered structure obtained by SLS. Then, it will be used to optimize the process parameters and the shapes of products in the design step. Up to now, the damage mechanisms in compounds obtained by SLS 3D printing are not very well understood, even less for products obtained from composite powders. The objectives imply several challenges related to the numerical simulation of complex crack networks in highly heterogeneous materials, the detection of micro cracks by 3D imagery imaging within combined with in situ mechanical testing, the modelling of damage and its identification at both micro and macro scales. The mechanical parameters, including the damage ones, will be characterized at the micro and macro scales by approaches combining tomography within microstructures (damage at the interfaces, damage related to the layered structure of the material) or at the scale of the workpiece, and numerical simulations through inverse approaches. The studied material is obtained from composite powder made of a polymer matrix of PA12 and containing glass beads. The powder is then sintered by laser to obtain 3D workpieces by PRISMADD. This project will allow optimizing the process parameters of the 3D process and the geometries of the workpieces with respect to failure criteria and lightweight. A numerical simulation code working able to capture damage mechanisms at both microscopic and macroscopic scales will be developed, based on the phase field method. This technique allows modelling initiation, propagation and merging of complex 3D crack networks in heterogeneous media. The method will be extended to the behaviour related to the material, characterized by a strongly nonlinear anisotropic behaviour. The tasks will consist into: (a) developing an efficient modeling numerical framework for simulating complex networks of cracks in highly heterogeneous microstructures from voxel models such as those arising from X-rays computed micro tomography imaging (XRµCT) and at the scale of the workpieces; (b) manufacturing by SLS 3D printing samples for a set of controlled process parameters; (c) characterize the strength properties of the new manufactured materials, with both macroscopic experimental mechanical testing and imaging at microscale, based on in situ mechanical testing in imaging devices and full-field kinematic measurement techniques, in 2D (optical observation) and in full 3D (XRµCT) ; (d) proposing microstructural and macroscopic damage models, identifying them by the mentioned experiments, and developing simplified multiscale damage models for bridging micro and macro damage; (e) optimizing the process parameters and the geometries of the produced workpieces with respect to the strength resistance of the produced products.

This project is devoted to the low-frequency (LF) vibration analysis of dynamical structures having a high modal density in the LF band. The increasing complexity of dynamical structures in many industrial fields (automotive, aerospace…) induces an increase of the LF modal density and requires new predictive and efficient tools for the analysis of their complex dynamical behavior. The frequency spectrum of such structures is characterized by the presence of well separated global elastic modes which are coupled with a large number of local elastic modes in this LF band. Therefore, the classical modal analysis, which is known to be efficient for the case of well-separated resonances is no longer adapted in the investigated case. Furthermore, the global elastic modes cannot easily be separated from the local elastic modes. Indeed, due to the coupling between global elastic modes and local elastic modes, the deformations related to global elastic modes include some local contributions. In the same way, the deformations related to local elastic modes include global contributions. Thus, there are no efficient method which can be used to select the global elastic modes and the local elastic modes. In addition, although the Reduced-Order Model (ROM) must be constructed with respect to the global elastic modes, it must have the capability to predict correctly the dynamical behavior of the structure in this LF range. Since there are local elastic modes in the LF range, a part of the mechanical energy is transferred from the global elastic modes to the local elastic modes. These local modes store this energy and then induce an apparent damping at the resonances associated with the global elastic modes. There are three objectives in this project: (1) the first objective concerns the construction of a robust ROM by using a basis which is constituted of global modes and which is able to take into account the effects of the local displacements. To achieve this objective we propose to use a recent method which allows the extraction of a basis of global displacements and a basis of local displacements by solving two separated eigenvalue problems. The reduced modeling of the local contributions is the main issue for which this project aims to provide a solution. (2) The second objective is to construct ROMs in the context of slender complex dynamical structures which are characterized by a high modal density of local elastic modes in the LF band. Usually, the industry uses equivalent beam models (for which the validity is generally limited to the first resonances) to analyze this type of structure. We propose here to directly extract beam-like vectors in order to construct a ROM, which remains predictive in a large frequency domain. (3) The third objective concerns the dynamical analysis of non-linear structures (large deformations). The use of the modal analysis method to reduce the non-linear equations is prohibitive when the modal density is too high. We propose here to construct a ROM by using a global displacement basis and if needed by taking into account the effect of the local displacements. These three objectives would be achieved through: - Theoretical developments. - Experimental validations on a simple structure. - Several industrial applications. Concerning the final phase, the first industrial application concerns the construction of an efficient ROM of an automotive vehicle in collaboration with PSA Peugeot-Citroën. The second one concerns an application for the fuel assembly of a Pressurized Water Reactor in collaboration with EDF R&D. These researches aim at removing methodological locks and at providing non-intrusive methods directly usable by the involved engineers.