Root2Res will deliver a package of solutions to enhance the resilience of rotational cropping system by considering relevant root traits with respect to the impact of climate change. Innovations will include phenotyping, genetic and modelling tools that will help breeders evaluate, in field and controlled conditions, novel and existing genotypes of a range of crops (Cereals, Potatoes, Legumes) as root ideotypes for different soil and climatic environments across Europe. Root2Res will also investigate the potential role of emerging crops (Sweet potato, Lentil) to enhance resilience to environmental change, by assessing their genotypic and phenotypic variation. The environments targeted include those predicted to suffer from the largest impact of climate change on yield in Europe. Resilience to stress will focus on greater variation in water availability (both drought and waterlogging) and interactions with other stresses (temperature, reduced nutrient availability). We will also consider the impact of novel understanding of the plasticity of traits to stress. Traits to be assessed will focus on exploration, exploitation and rhizosphere microbiome related traits integrated in an extended phenotype. The impacts of the more resilient ideotypes designed in Root2Res on the delivery of climate change mitigation outcomes (soil carbon sequestration, nutrient utilisation and greenhouse gas emission) will be assessed in field. Root2Res will integrate a strong interaction with stakeholders all through the project, particularly breeders and farmers. The ambition of Root2Res is to deliver crops adapted to changing environments and able to mitigate climate change, by utilising existing genetic diversity for breeding programs in a range of crop species essential to cropping systems and then widening understanding to crops suitable for resilient future systems. This ambition will be supported by the joined commitment of a multidisciplinary partnerships across Europe and beyond.

Food production is predicated on the application of nitrogen fertilisers, which can contribute significantly to the production of greenhouse gasses and eutrophication of agroecosystems. The use of nitrogen fertilisers must, therefore, be optimised. The recent development of transparent soils in my group gives great scope to unravel the processes involved in the reactive transport of nutrients in soil and their interaction with the soil biota. My team will combine principles of optics, chemical engineering, the physics, chemistry, and biology of soils, and plant biology to image and characterise nitrogen movement in soil at the micro-scale. We will develop a new generation of transparent soil analogues that measure the biological and chemical status of soils. This will enable, for the first time, to characterise transport at the surface of soil particles and to elucidate the role of root–particle–particle contacts, exudation and microbial transformation on the bioavailability of nitrate and ammonium. The legacy of the research will be knowledge, concepts, model soil systems and imaging approaches to understand and predict nutrient bioavailability in soil with an emphasis on nitrification as a model for nitrogen movement in soil. Transparent soils and imaging technologies will be patented and could pave the way for 3D chemical sensors, and have application in crop breeding and precision phenotyping. Understanding of nutrient movements in soil will lead to substantial progress in the development of more efficient fertilisers. New model soil systems could be used to better understand the spread of soil-borne diseases, the bio-remediation of contaminated soils and the mechanisms underlying soil biodiversity and activity.

Agriculture is currently confronting (i) an increasing human population and (ii) limitations of soil use due to, among other reasons, pollution levels above food safety threshold values. Some agricultural practices increase the heavy metal content (HM) of agricultural soil, representing an important threat for the European agricultural development. The use of microorganisms as plant growth promoters has been increasingly studied for a number of years, but it has only recently been proposed to improve plant metal tolerance. Regrettably, plant-microorganism-pollutant interactions are still poorly understood and the molecular underlying mechanisms are mostly unknown. The abovementioned challenges for agricultural production require the study of these mechanisms to better promote a more efficient and sustainable agriculture. This project will venture into new unchartered territory by focusing on the molecular interactions between a probiotic actinobacterium (Micromonospora cremea) and its host, Pisum sativum (garden pea), in the presence of HMs. We will evaluate the capacity of M. cremea CR30 to improve plant tolerance to HM polluted soils, in addition to unraveling the molecular dialogue during the first and late steps of their interaction. Early step interactions are crucial in plant promotion and protection against external stresses, like pollution by HM. Here, we propose the use of new -omic technologies to study these molecular interactions between plants and microorganisms under metal stress, providing a new pathway for an improved soil management. This project addresses a crucial objective in food security, the development of sustainable agricultural practices to control potentially adverse HM effects on plant health.

LANDFEED will focus on creating value from under-utilised waste from the agro-food industry, forestry, urban and natural waste, implementing circular and local solutions that allow waste to be valorised by placing it in a circular framework, and producing innovative biofertilisers to improve Europe's self-sufficiency. In addition to optimising and implementing innovative nutrient recovery technologies, work will be carried out on a new generation of coatings for these bio-based fertilisers, capable of improving their efficiency through controlled nutrient release mechanisms. In this way LANDFEED will contribute to a better management of the fertiliser provided, we will contribute to lower greenhouse gas emissions and a reduced impact on the environment's water resources. LANDFEED will ensure that the solutions and results of the project are locally driven through the different use cases. The use cases will consider all links in the value chain that will participate as lighthouses, serving as demonstrators and disseminators of the technologies, results and applications developed during the project. These use cases will also contribute to the objectives of the Soil Strategy by enabling the restoration of soil health through the enhancement of its specific and functional biodiversity. At the global level, the business model will be defined in its entirety, with the aim of maximising the replicability of these Use Cases and facilitating their implementation in other European areas and regions.