Antibiotics are used extensively to fight bacterial infections and have saved millions of lives. However, the bacteria are becoming resistant to antibiotics and some antibiotics have stopped working. We refer to this as antimicrobial resistance - AMR. We don't just use antibiotics for people; similar amounts are given to farm animals. More than 900 million farm animals are reared every year in the UK and antibiotic treatments are vital for their welfare, for farms as businesses, and for us to enjoy affordable food. However, farms may be contributing to the development of AMR. The aim of this project is to improve our understanding of how farm practice, especially the way in which manure is handled, could lead to AMR in animal and human pathogens. This understanding will help farmers and vets find new ways to reduce AMR, without harming their animals or their businesses. For research purposes, Nottingham University maintains a typical high performance dairy farm - its 200 cows produce a lot of milk and a lot of manure. The waste is stored in a 3 million litre slurry tank, any excess goes into a 7 million litre lagoon. This slurry is applied to fields as organic fertilizer. Cow manure contains many harmless bacteria but some, e.g. E. coli O157, can cause severe infection in people. When cows get sick they are treated with antibiotics. Udder infections are treated by injection of antibiotics into the udder. Since this milk contains antibiotics, it cannot be sold but is discarded into the slurry. Foot infections are treated with an antibacterial footbath, which is also emptied into the slurry tank. As a result, slurry tanks contain a mixture of bacteria, antibiotics and other antimicrobials that are stored for many months. The bacteria that survive in the presence of antibiotics are more likely to have antibiotic resistance. This resistance is encoded in their genes so passed to the next generation. Worse still, the genes can be passed on to other bacteria in the slurry. Before we wrote this proposal, we investigated our own farm's slurry tank to see if this might be happening. We tested 160 E. coli strains from the slurry; most carried antibiotic resistance. We also found antibiotics in the tank - including some that bacteria were resistant to. Our mathematical modellers showed that reducing spread of resistance genes in the tank might be more effective in preventing resistance than cutting the use of antibiotics. Conversations with the farm vets revealed that they knew about AMR and had changed some of their antibiotic prescriptions. But these analyses leave us with more questions than answers. In this project, we want to find out if current farming methods are contributing to the development of harmful antibiotic-resistant bacteria in slurry, bacteria that may then be encountered by humans and animals. To do this, we need to integrate scientific and cultural approaches: - What bacteria are in the slurry? How many are harmful? What resistance genes do they carry? How do these genes spread? - How long do antibiotics remain in the tank? Do they degrade? - What happens to the bacteria and antibiotics after they are spread on fields? - How do farmers, vets and scientists interpret evidence about AMR? What are their hidden assumptions? Can we improve collaborative decision making on AMR risk management? - Can we reduce resistance by avoiding mixing together bacteria and antimicrobials in slurry? - Can we predict the risk of emergence of and exposure to resistant pathogens? Can we predict which interventions are likely to be most effective to reduce AMR, taking into account both human and scientific factors? Through this research, we will learn what can realistically be done to reduce this risk; not just on this farm, but UK wide. We will work with farmers, vets and policy makers to ensure that our results will make a difference to reducing the risk of harmful AMR bacteria arising in agriculture.

The global hydrogen generation market is valued at $115.25 billion in 2017 and is projected to grow to $154.74 billion by 2022 [Global Outlook & Trends for Hydrogen, IEA, 2017]. We are witnessing significant market opportunities emerging for hydrogen technologies today. New and existing hydrogen technology developments and market activities are projected to intensify over the coming decade. Sustainable hydrogen solutions are a key pathway for decarbonising transport, heat and power generation sectors. Common challenges to sustainable hydrogen being adopted across these sectors are: - Cost reduction - Safety - Systems level and multisectoral innovations - Managing change Over the next decade innovative solutions are needed to tackle the above challenges, but it will be impossible without a dedicated mechanism to train doctoral Energy Innovation Leaders. These leaders should have a firm grasp of the technology from scientific fundamentals through to applied engineering and a solid understanding of the techno-economic barriers and an appreciation of the societal issues that will impact on the translation of disruptive technologies from research labs through to market. This goes beyond being multidisciplinary, but is a transdisciplinary training, reflecting the translation steps from understanding market driven needs, planning and conducting appropriate basic and applied research to products/solutions/system development through to successful market penetration. This is delivered by a cohort training approach through the cross fertilisation of ideas of a cohort with a diverse background, peer-demonstration of the value of research across a diverse range of stakeholder-led projects, thus facilitating a peer-to-peer transdisciplinary learning culture. The SusHy Consortium, led by Gavin Walker, continues a long running and highly successful collaboration in hydrogen research between the Universities of Nottingham, Loughborough, and Birmingham (UoN, LU, UoB) which started over a decade ago with the Midlands Energy Consortium. The Midlands Energy Graduate School spawned two successful CDTs (Hydrogen, Fuel Cells and their Applications and the current Fuel Cells and their Fuels). The current proposal for a CDT in Sustainable Hydrogen brings together the world leading expertise in hydrogen generation, purification, sensors/monitoring, and storage, along with whole systems issues (resilience engineering, business economic models and life cycle analysis) which exist across the three Universities. A gap in the consortium expertise is in the research field of hydrogen safety and we identified the internationally-renowned Hydrogen Safety Engineering and Research Centre (HySAFER) at Ulster University (UU) as the right partner to deliver on this key aspect. This is the first broad collaboration in the world seeking to investigate, train researchers and produce leaders in Sustainable Hydrogen. Stakeholder Partnerships. A key strength of this CDT is the active involvement of the Stakeholders in co-creation of the training programme which is reciprocated in the value with which the Stakeholders view of the CDT. This shared vision of a training partnership between the Universities and Stakeholders will lead to the smooth function of the CDT with not just a high-quality training programme, but a programme that is tailored to the sector needs for high-quality, industry-ready doctoral Energy Innovation Leaders. The valued CDT-stakeholder partnership will also be a significant appeal to candidates interested in energy-related PhDs and will be used to help market the CDT programme to a diverse talent pool.