A new method for minimizing the spread of bioeffluents emitted from hospitalized patients lying in beds was developed and studied. The method consists of a ventilated bed mattress that is able to exhaust the human bioeffluents at the area of the body where generated before spreading around in room. Full-scale experiments were conducted in a climate chamber furnished as a two-bed hospital patient room. A thermal manikin and two heated dummies were used to simulate two lying patients and a standing doctor. The bed with the thermal manikin had the ventilated mattress (VM). The tracer gases CO2 and N2O were used to mimic human bioeffluents released from the feet and armpits of the manikin, respectively. The concentration of the tracer gases was measured in six points including the breathing zone of the simulated occupants. The results show that the VM combined with mixing ventilation at 1.5 air changes per hour (ACH) proved to be more effective in reducing exposure to body contaminants compared to mixing ventilation alone at 3 ACH and 6 ACH. The findings also show that the lying position and the size of the local exhaust of the VM affect the efficiency of the mattress to exhaust bioeffluents.