Biodiversity loss in hotspots of biodiversity is, among other socio-ecological factors, key to understand, prevent and react to future pandemics. However, despite this knowledge, the current COVID-19 crisis highlights the limitations of the implementation of One Health approaches. A main limitation is the lack of context-adapted solutions that stakeholders (national authorities but also local communities, NGOs and private companies) could easily implement on the field. To overcome this, the MARBLES partners will build on past international projects to co-construct innovations with all stakeholders of biodiversity hotspots to reduce the risk of infectious disease emergence through biodiversity conservation and disease surveillance strategies. The activities of the project will be implemented in Europe and three tropical biodiversity hotspots in Southeast Asia, West Africa and Central America and will have the following expected impacts: • the results of the MARBLES project will lead to a better understanding of the mechanisms underlying the impact of biodiversity on the risk of infectious disease emergence • Stakeholder’s engagement tools developed during the MARBLES project will facilitate the design of context-adapted biodiversity conservation and restoration strategies that reduce zoonotic risk • the surveillance strategies and pathogen detection tools developed during the MARBLES project will improve the capacities to detect emergences and stop future epidemics before they can turn into pandemics The consortium will constitute a strong multi-actor group of partners with a history of successful cooperation including academics from biomedical, environmental and social sciences, private companies, NGOs, local and international stakeholders who bring together the wide range of disciplines and expertise required to reach all the expected outcomes of the HORIZON-CL6-2021-BIODIV-01-11 call. Overall, the implementation of the MARBLES project in various ecological, climatic and cultural settings will allow the consortium members to develop generic innovations that could subsequently be adapted through to a wide range of socio-ecological settings, contributing to the understanding, prevention and early detection of zoonotic emerging threats globally. The embedment of the MARBLES project in the Prezode initiative will help to scale up the project innovations and disseminate cutting-edge socio-economic environmental strategies.