Type 2 Diabetes (T2D) is the main epidemic of this century. A recent hypothesis of medical research is that an important cause of T2D may be the abnormal regulation of glucose absorption in the small intestine. The objective of the present project is to investigate the relative contribution of each regulatory mechanism in the postprandial glucose response, with a particular focus on the mechanism of intestinal glucose absorption. Indeed, despite of many experimental observations, this question remains poorly investigated. Both whole-body physiological and cellular level will be considered. We will adopt a systems biology approach based on formal computational models enhanced by wet-lab experiments. Unlike all the models already available and defined by means of differential equations, we will propose to work with reaction networks. Those are metamodels that add a graph structure to ordinary differential equations (ODEs) and allow for a wider range of analysis methods from computational systems biology. We will also study novel analysis methods for reaction networks able to deal with aspects of postprandial glucose response and diabetes. The hope is to improve the identification of the causes of T2D and, in the longer run, the prediction of appropriate therapies based on reaction networks.