CapaCITIES 2.0 is designed as a support action for national and regional authorities to advance their governance structures for providing dedicated support for cities to achieve the EU Climate-Neutral and Smart Cities Mission (EU Cities Mission) - answering to the Call HORIZON-MISS-2024-CIT-02-01. As a central initiative to foster and strengthen national narratives for the EU Cities Mission, it will coordinate actions across climate-neutral initiatives for cities in Europe, align and speed up efforts towards the goals of the Mission. CapaCITIES 2.0 will further shape a European environment conducive to urban climate neutrality transitions for national, regional and local authorities. It will initiate and strengthen national change processes to establish and mature national networks and platforms as well as multi-level governance in the entire EU, building on the experience of the CapaCITIES that already started setting-up national platforms in 15 EU countries. It provides dedicated support for public authorities to put enabling conditions and measures for cities in place to achieve the EU Cities Mission. Thereby, it contributes to reach (1) Increased preparedness and capacity of national, regional and local authorities in EU Member States and countries associated to Horizon Europe to engage in cities’ transition towards climate neutrality; (2) Improved ability to effectively address country-specific challenges and barriers for cities to achieve climate neutrality related to current regulatory frameworks, funding and financing, and governance structures and promotion of best-practices through transnational exchanges and sharing of experience and (3) Enhanced synergies with R&I national/international communities, relevant initiatives, and partnerships such as the ‘Driving Urban Transitions to a Sustainable Future’ Horizon Europe Partnership and the NetZeroCities – the EU Mission Platform(NZC) for the implementation of the Climate-Neutral and Smart Cities Mission.