The DUT Partnership builds upon and steps up the ambition and efforts of the JPI Urban Europe. With its partners from 28 countries it aims at developing and implementing a transformative research and innovation (R&I) programme to strengthen urban transitions towards climate neutral, inclusive and sustainable urban areas. With national and regional R&I programme owners, funders and urban policy related public authorities, DUT is strongly building upon and aligning with national and regional strategies and policies. In order to support cities along their specific strategies, the Partnership focuses on three critical urban sectors, energy, mobility and circular economy, and their interrelationships. Three Transition Pathways – Positive Energy Districts, the 15-Minute City and Circular Urban Regenerative Economies – will be addressed with a long-term perspective. DUT will not only provide funding for transnational R&I projects in and across these areas through implementing joint calls but establish a comprehensive programme management to support capacity building and enhance the (policy) impact of these projects and their results. In this regard, programme activities will address capacity building and empowerment of all urban stakeholder groups to engage in urban research and innovation and codesign solutions. Urban living labs will be fostered as an important concept to support local experimentation and inter- and transdisciplinary collaboration. New approaches towards dissemination and mainstreaming of research results will be developed with the ambition to bring results into our cities. Strategic synthesis as well as recommendations and references for policy making will be provided with particular focus on contributions to the Urban Agenda for the EU, the European mission for climate-neutral and smart cities and the European Green Deal. Efforts will be taken to position the DUT partnership internationally and mobilise international partners to join forces in DUT.