The NERC Biomolecular Analysis Facility provides access to high-level genomics, metabolomics and bioinformatics provision through its five nodes. NBAF offers the very latest, class-leading technologies, including next-generation sequencing (Roche 454, Illumina and SOLiD), Sanger sequencing, microarray- and sequencing-based genotyping and expression profiling including the in silico design of oligoarrays (Agilent, Nimblegen). NBAF also supports metabolomics, medium-scale genotyping, bioinformatics and advanced data analysis techniques (genome and transcriptome assembly and annotation, expression analysis, etc.). The Facility consists of five nodes: a) NBAF-Sheffield - molecular genetics generation and analyses of molecular markers for population and evolutionary genetics; b) NBAF-Liverpool - high-throughput sequencing and microarray analysis technologies used for gene expression analysis, sequence capture, next-generation sequencing using Roche 454, AB SOLiD4 & IonTorrent technology, and bioinformatic support for analyses of these data; c) NBAF-Edinburgh - high-throughput sequencing and genomic analysis using Sanger, Roche 454 and Illumina technology, and bioinformatic support for analyses of these data; d) NBAF-Birmingham - metabolomics training, assay and analysis; e) NBAF-Wallingford - bioinformatics core training, bespoke analyses, and integrative analyses.