Abstract Background and Aim: Increasing energy consumption has created an energy crisis in the world. Fossil fuels are limited and depleting. Biogas is considered a fuel that has attracted the attention of researchers. To increase biogas production, different pretreatments have been utilized. The purpose of this study was to investigate the optimal mixing ratio of Municipal Solid Waste (MSW) and Sewage Sludge (SS), as well as the effects of various conditions of alkaline pretreatment on biodegradability of wastes and the amount of biomethane production. Materials and Methods: This study was done in a laboratory digester with 1 L volume at 37 °C with different concentrations of NaOH in a completely randomized design. Biogas volume, methane volume, and changes in pH were measured daily. Measurement parameters in the anaerobic digestion including total solids, volatile solids, and carbon and nitrogen content in the feedstock were determined according to the APHA standard methods. Results: The optimal mixing ratio of MSW to SS was 60:40 with the highest methane yield of 254.87 mL/g VS. Next, the effects of 2, 6, and 10% NaOH concentrations were evaluated on the amount of gas produced, indicating that 6% NaOH concentration significantly improved waste decomposition. Methane production, VS, and TS removal were compared to the control treatment, and there were increases of 30, 27.94, and 27.25%, respectively. Conclusion:The results showed that the mixing ratio of MSW to SS at 60:40 with 6% NaOH improves the decomposition of organic wastes and increases biomethane production. Keywords: Alkaline Pretreatment; Anaerobic Digestion; Biogas; Municipal Solid Waste; Sewage Sludge