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Statistical study of the growth kinetics and lipid content of microalgae cultivated in brackish media, using wastewater as a source of nutrients for bioenergetic purposes

Authors: Guimarães, Bruna da Silveira; França, Kepler Borges; Nascimento, João Alexandre Rodrigues; Dantas, Carolina Pereira;

Statistical study of the growth kinetics and lipid content of microalgae cultivated in brackish media, using wastewater as a source of nutrients for bioenergetic purposes

Abstract

El presente trabajo tuvo como objetivo probar estadísticamente la influencia de la concentración de nutrientes de aguas fósiles sobre la cinética de crecimiento y acumulación de lípidos intracelulares en microalgas. Se estudiaron las especies Chlorella sp., Scenedesmus acuminatus, Nannochloropsis sp., Monoraphidium contortum y Pediastrum tetras, que fueron aisladas de la región semiárida del Nordeste brasileño y cultivadas en una mezcla compuesta de aguas subterráneas salobres y efluentes de fosas sépticas. De los cultivos se analizaron los parámetros cinéticos, producción de biomasa seca y contenido de lípidos, utilizando como herramienta el diseño experimental para un factor, con la concentración de nutrientes como variable independiente. La velocidad máxima de crecimiento (μmax), el tiempo de generación (tg), así como el contenido de lípidos fueron las variables de respuesta estudiadas. Los resultados mostraron que las especies que presentaron mayor acumulación de lípidos fueron Chlorella sp., con un contenido de lípidos de 81,20% y Nannochloropsis sp. con un 73,68%, siendo el más promisorio para fines bioenergéticos. Por lo tanto, la concentración de nutrientes de los efluentes fósiles combinada con la salinidad del medio actuó directamente sobre el crecimiento celular y el contenido de lípidos, y estos factores pueden utilizarse como un dispositivo para mejorar la producción de lípidos, con el objetivo de producir biocombustibles.

O presente trabalho teve como objetivo comprovar, estatisticamente, a influência da concentração de nutrientes provenientes de águas fóssicas na cinética de crescimento e acumulação lipídica intracelular em microalgas. Foram estudadas as espécies Chlorella sp., Scenedesmus acuminatus, Nannochloropsis sp., Monoraphidium contortum e Pediastrum tetras, as quais foram isoladas da região semiárida nordestina brasileira e cultivadas na mistura composta por água subterrânea salobra e efluente oriundo de fossas sépticas. A partir dos cultivos foram analisados os parâmetros cinéticos, produção de biomassa seca e teor de lipídeos, utilizando como ferramenta o planejamento experimental para um fator, tendo a concentração de nutrientes como variável independente. A velocidade máxima de crescimento (μmáx), tempo de geração (tg), bem como o teor de lipídeos foram as variáveis resposta estudadas. Os resultados mostraram que as espécies que apresentaram maior acúmulo de lipídios foram Chlorella sp., com conteúdo lipídico de 81,20% e Nannochloropsis sp. com 73,68%, sendo as mais promissoras para fins bioenergéticos. Portanto, a concentração de nutrientes provenientes de efluentes fóssicos aliada à salinidade do meio atuaram diretamente no crescimento celular e teor de lipídeos, podendo tais fatores serem utilizados como artifício para potencializar a produção lipídica, visando a produção de biocombustíveis.

The present work aimed to prove, statistically, the influence of the concentration of nutrients from fossil waters on the growth kinetics and intracellular lipid accumulation in microalgae. The species Chlorella sp., Scenedesmus acuminatus, Nannochloropsis sp., Monoraphidium contortum and Pediastrum tetras were studied, which were isolated from the semiarid region of the Brazilian northeast and cultivated in a mixture composed of brackish groundwater and effluent from septic tanks. From the crops, the kinetic parameters, dry biomass production and lipid content were analyzed, using the experimental planning for one factor as a tool, with the concentration of nutrients as an independent variable. The maximum growth velocity (μmax), generation time (tg), as well as the lipid content were the studied response variables. The results showed that the species with the greatest accumulation of lipids were Chlorella sp., with a lipid content of 81.20%, and Nannochloropsis sp. with 73.68%, being the most promising for bioenergetic purposes. Therefore, the concentration of nutrients from fossil effluents combined with the salinity of the medium acted directly on cell growth and lipid content, and such factors can be used as an artifice to enhance lipid production, aiming at the production of biofuels.

Keywords

Biotecnología, Biomasa, Reuse, Biomassa, Lípidos., Águas Salobras, Biotecnologia, Biocombustibles, Agua salobre, Lipídeos., Lipids., Reuso, Biofuels, Biocombustíveis, Brackish Water, Biomass, Reutilización, Biotechnology

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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