Forests are an increasingly important source of feedstock for bioenergy as global efforts to mitigate atmospheric CO2 concentrations increase. In keeping with the principles of sustainable forest management, it is important that feedstock procurement not have negative impacts on the environment, including biodiversity. Impacts of land use, including forest management, can be evaluated along all stages in the production of these goods and services, using life cycle assessment (LCA), which is a potentially powerful tool for organizing and evaluating the impacts of production. There is growing recognition of the need to integrate land‐use impacts into LCA for forest products such as bioenergy, especially on biodiversity. Integrating quantitative indicators of biodiversity into LCAs of biomass production systems is particularly challenging because biodiversity is a multidimensional concept that can never be fully represented by a single number, and yet many proposed approaches rely on this. Reliance on a single metric oversimplifies ‘biodiversity’ and might lead to inappropriate conclusions on local land management practices. LCA is not suited to providing reliable site‐specific assessment of forest product systems in regard to the complexities of biodiversity. Nevertheless, the global and comprehensive nature of LCA makes it a useful tool for preventing a shift in environmental problems or burdens across the value chain because of local land management decisions. In this context, complementary site‐specific and/or regional studies or analyses may help mitigate against inaccurate conclusions being drawn from LCA. WIREs Energy Environ 2016, 5:670–683. doi: 10.1002/wene.211This article is categorized under: Bioenergy > Climate and Environment Energy and Development > Climate and Environment