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Photosynthesis Research
Article . 2018 . Peer-reviewed
License: CC BY
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Photosynthesis Research
Article
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Spectrally decomposed dark-to-light transitions in Synechocystis sp. PCC 6803

Authors: Alonso M. Acuña; Pascal van Alphen; Filipe Branco dos Santos; Rienk van Grondelle; Klaas J. Hellingwerf; Ivo H. M. van Stokkum;

Spectrally decomposed dark-to-light transitions in Synechocystis sp. PCC 6803

Abstract

Photosynthetic activity and respiration share the thylakoid membrane in cyanobacteria. We present a series of spectrally resolved fluorescence experiments where whole cells of the cyanobacterium Synechocystis sp. PCC 6803 and mutants thereof underwent a dark-to-light transition after different dark-adaptation (DA) periods. Two mutants were used: (i) a PSI-lacking mutant (ΔPSI) and (ii) M55, a mutant without NAD(P)H dehydrogenase type-1 (NDH-1). For comparison, measurements of the wild-type were also carried out. We recorded spectrally resolved fluorescence traces over several minutes with 100 ms time resolution. The excitation light was at 590 nm so as to specifically excite the phycobilisomes. In ΔPSI, DA time has no influence, and in dichlorophenyl-dimethylurea (DCMU)-treated samples we identify three main fluorescent components: PB-PSII complexes with closed (saturated) RCs, a quenched or open PB-PSII complex, and a PB-PSII 'not fully closed.' For the PSI-containing organisms without DCMU, we conclude that mainly three species contribute to the signal: a PB-PSII-PSI megacomplex with closed PSII RCs and (i) slow PB → PSI energy transfer, or (ii) fast PB → PSI energy transfer and (iii) complexes with open (photochemically quenched) PSII RCs. Furthermore, their time profiles reveal an adaptive response that we identify as a state transition. Our results suggest that deceleration of the PB → PSI energy transfer rate is the molecular mechanism underlying a state 2 to state 1 transition.

Countries
Netherlands, Netherlands
Keywords

Time-resolved spectroscopy, 570, Synechocystis/physiology, Light, Energy Transfer/physiology, Plastoquinone pool, Plant Science, Cyanobacteria, Biochemistry, Phycobilisomes/metabolism, Thylakoids, Photosystem II Protein Complex/metabolism, Fluorescence, Electron Transport, Thylakoids/metabolism, Photosystem I Protein Complex/metabolism, Phycobilisomes, Photosynthesis, 580, Photosystem I Protein Complex, Spectrometry, Singular value decomposition, Bacterial, Synechocystis, Photosystem II Protein Complex, Cell Biology, Gene Expression Regulation, Bacterial, Cyclic electron flow, Spectrometry, Fluorescence, Spectrally resolved fluorometry, Gene Expression Regulation, Energy Transfer, Electron Transport/physiology, Photosynthesis/physiology

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
4
Average
Average
Average
Green
hybrid