Abstract The AP1000 ® is an advanced Pressurized Water Reactor (PWR) design developed by Westinghouse which implements passive safety systems to provide core cooling in case of accident. The development of best-estimate codes produced the evolution of conservative safety analysis towards the so-called best-estimate plus uncertainty (BEPU) analysis in order to obtain more realistic results and larger safety margins. In this sense, Westinghouse used for AP1000 Large Break Loss of Coolant Accident (LBLOCA) the so-called Automated Statistical Treatment of Uncertainty Method (ASTRUM) which was developed to address this kind of BEPU analysis. This paper presents a verification of the AP1000 LBLOCA BEPU analysis by means of TRACE V5.0 patch 2 thermal–hydraulic code with the support of DAKOTA code for uncertainty calculations. The results obtained show lower values for the maximum PCT than the ones obtained by Westinghouse. In both cases the results show that AP1000 can mitigate effectively the occurrence of a postulate LBLOCA and to meet the 10CFR50.46 PCT acceptance criteria with enough margin.