Abstract In this paper, a double-acting thermoacoustic Stirling electric generator is proposed as a new device capable of converting external heat into electric power. In the system, at least three thermoacoustic Stirling heat engines and three linear alternators are used to build a multiple-cylinder electricity generator. In comparison with the conventional thermoacoustic electricity generation system, the double-acting thermoacoustic Stirling electric generator has advantages on efficiency, power density and power capacity. In order to verify the idea, a prototype of 3 kW three-cylinder double-acting thermoacoustic Stirling electric generator is designed, built and tested. Based on the classic thermoacoustic theory, numerical simulation is performed to obtain the thermodynamic parameters of the engine. And distributions of key parameters are presented for a better understanding of the energy conversion process in the engine. In the experiments, a maximum electric power of about 1.57 kW and a maximum thermal-to-electric conversion efficiency of 16.8% were achieved with 5 MPa pressurized helium and 86 Hz working frequency. However, we find that the mechanical damping coefficient of the piston is dramatically increased due to the deformation of the cylinder wall caused by high thermal stress during the experiments. Thereby, the system performance was greatly reduced. Additionally, the performance differences between three engines and three alternators are significant, such as the heating temperature difference between three heater blocks of the engines, the piston displacement and the output electric power differences between three alternators. These problems need further investigation. This work presents a new thermal-to-electric conversion technology, which can be utilized in many energy area, such as solar energy, industrial waste heat and so on.