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Real time light intensity based carbon dioxide feeding for high cell-density microalgae cultivation and biodiesel production in a bubble column photobioreactor under outdoor natural sunlight

pmid: 30925422
Outdoor high cell-density microalgae cultivation is highly challenging due to unavailability of appropriate CO2 feeding strategy under diurnal sunlight intensities. Hence, a novel real time light based CO2 feeding strategy was firstly developed under diurnal simulated sunlight (LED) to test on Chlorella sp. in a 10 L scale bubble column photobioreactor. The strategy yielded a biomass titer of 5.12 g L-1 under simulated sunlight, far higher than existing biomass-density and pH-control based CO2 feeding strategies. In outdoor culturing, the proposed feeding strategy yielded high biomass titers of 6.8 and 9.0 g L-1 in growth-phase of two-stage and single-stage lipid induction studies respectively with same biomass productivity of 0.8 g L-1 day-1. Subsequently, two-stage lipid induction strategy of 6.8 g L-1 titer yielded biodiesel productivity of 120 g L-1 day-1, whereas single-stage strategy of 9.0 g L-1 titer was unable to induce lipid. Moreover, specific light availability affects the lipid production.
Chlorella, Carbon Dioxide, Lipids, Photobioreactors, Biofuels, Microalgae, Sunlight, Biomass
Chlorella, Carbon Dioxide, Lipids, Photobioreactors, Biofuels, Microalgae, Sunlight, Biomass
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