Wastewater with 0.2, 0.4, 0.8, 1.0 mg/L free chlorine was biologically treated using co-immobilized microalgae/bacteria. In contrast, non-pretreated wastewater was treated with beads (control) and blank beads (blank) under the same operating condition. Results showed that NaClO pretreatment removed 8-33% total nitrogen (TN), 31-45% true color and 0.7-2.5 log CFU/mL aerobic-bacteria. At the end of treatment, maximum algal biomass (2,027 dry weight mg/L) was achieved with 0.2 mg/L free chlorine. Bacterial growth in wastewater was decreased by NaClO pretreatment before reaching 7.2-7.7 log CFU/mL on the fifth day. Beads with microorganisms (control) removed 15% more chemical-oxygen-demand (COD), 16% more TN, and 13% more total phosphate (PO43-) than blank. Pretreatment with 0.2 mg/L free chlorine increased TN removal from 75% to 80% while pollutants removal was substantially decreased with 0.4-1.0 mg/L free chlorine. Considering algal biomass growth and pollutants removal, 0.2 mg/L free chlorine pretreatment was recommended for microalgae/bacteria co-immobilized system.