Continuous automation of conventional industrial operations with smart technology have drawn significant attention. Firstly, the study investigates on optimizing the proportion of industrial biscuit processing waste powder, (B) substituted into BG-11 as a source of cultivation medium for the growth of C. vulgaris. Various percentages of industrial biscuit processing waste powder, (B) were substituted in the inorganic medium to analyse the algal growth and biochemical composition. The use of 40B combination was found to yield highest biomass concentration (4.11 g/L), lipid (260.44 mg/g), protein (263.93 mg/g), and carbohydrate (418.99 mg/g) content compared with all the other culture ratio combination. Secondly, the exploitation of colour acquisition was performed onto C. vulgaris growth phases, and a novel photo-to-biomass concentration estimation was conducted via image processing for three different colour model pixels. Based on linear regression analysis the red, green, blue (RGB) colour model can interpret its colour variance precisely.