AbstractThe potentiality in biogas production from anaerobic co-digestion of canned seafood wastewater (CSW) with glycerol waste (GW) was investigated. Methane yields from anaerobic co-digestion of CSW with 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10% (v/v) of GW were 577, 265, 101, 51, 11, 9, 4, 3, 2 and 2mLCH4/g VS-added, respectively. The anaerobic co-digestion of 99% CSW with 1%GW was the optimal mixture ratio for methane production with a maximum methane yield was 577mLCH4/g VS-added and 97% biodegradability. Meanwhile, the maximum methane yield of 1%GW and 100%CSW were 211 and 278mLCH4/g VS-added. Methane yield increased by 108% when compared with digested CSW alone. The maximum methane production from anaerobic co-digestion of 99%CSW with 1%GW was 5.8 m3 CH4/m3 of mixed wastewater and electricity production of 1 m3 mixed wastewater would be 207MJ or 58 kWh of electricity. Continuous methane production from anaerobic co-digestion of CSW with 1% GW in up-flow anaerobic sludge blanket (UASB) reactors gave methane production rate of 2.33 LCH4/L-reactor.day.