Abstract This study was carried out to analyze contrastively two modes of air conditioning with the bottom-supply and the stratum ventilation by combined the numerical simulation with the physical experiment in the laboratory. The changing rules of indoor thermal environment, human body thermal comfort and energy utilization efficiency under two kinds of air supply mode were explored. The mode of the supply air with the better distribution of indoor thermal environment and the better human comfort was gained to meet the requirements of green air conditioning for the human. The studied results showed that, according to the evaluation index of human thermal comfort and the energy utilization efficiency, the mode of bottom-supply air conditioning was better than the mode of stratum ventilation. So it might be suitable to select the mode of bottom-supply in the design of air conditioning engineering for office buildings to meet the requirements of human on indoor comfort air-conditioning.