Abstract Indoor comfort perception of buildings occupants depends on several parameters related to physical boundary conditions but also to the adaptation capability of occupants themselves. According to standards, just physical ambient parameters are considered to evaluate comfort so non-measurable factors, such as psychological ones, are not taken into account. The present work aims to identify possible benefits in terms of occupants’ comfort perception due to the maintenance of good quality work environment. To this purpose, the environmental multi-physics performance of a mixed industry-office building is investigated through both field microclimate monitoring and questionnaires campaigns. Results obtained are therefore compared and discussed.