The present study applies an approach to analyze the stress induced on people on the autism condition. Dedicated questionnaires were prepared, in order to guarantee an inclusive participation and then applied by means of two different surveys in living and extended care unit environments. Results permit to highlight that: (i) the four comfort domains show similar sensitivity trends, with acoustics having the greatest impact on people with autism spectrum disorder (ASD), while thermo-hygrometric, visual and IAQ sensitivity decreases as the stress scale increases; (ii) Severity of autism influenced the acoustic sensitivity, while age and the considered environment influenced thermo-hygrometric, visual and IAQ sensitivities. The proposed approach can be applied to well-being studies involving people with other types of conditions which could alter their perception of the built environment.