The sn-2 monoacylglycerol (MAG) of eicosapentaenoic acid (EPA) can be used as a health-promoting ingredient in functional foods. However, the lack of a good recovery method to prepare high-purity 2-EPA MAGs has limited their application. In this study, circular ethanolysis and glycerolysis were repeated three times to synthesize 2-EPA MAGs and obtain a high recovery of EPA in them. Ethanolysis was carried out using 12 % of Lipozyme 435 and an ethanol-to-triacylglycerol (TAG) ratio of 60, which led to a TAG conversion rate of 97.3 %. Glycerolysis was then carried out using 16 % of CL "Amano" IM and a substrate-to-glycerol ratio of 9 (under vacuum), which led to a conversion rate of ethyl ester to TAGs of 96.8 %. After three ethanolysis-glycerolysis cycles, a relatively high recovery of EPA in the 2-MAG (72.1 %) was obtained. After purification, a high purity 2-EPA MAG product (EPA: 92.2 ± 1.8 %; 2-MAG: 91.50 ± 0.14 %) was obtained.