Blockchain is seen as a disruptive fundamental technology that will transform agri-food sectors in the near future. Blockchain is a digital, immutable, decentralized ledger of transactions that is replicated and distributed throughout the chain of computer systems on the blockchain's network. This systematic review examines the literature to identify the enabling characteristics of blockchain technology that support its application in the agri-food sectors. The literature reviewed indicated essential characteristics such as transparency, immutability, redundancy, versatility, automation, and remittance. Among these features, immutability and automation have a salient role in the current implementation, particularly in food traceability. However, the application of blockchain in the agri-food sector is not without controversies. More research is needed regarding technical improvement and its environmental impact.