
You have already added 0 works in your ORCID record related to the merged Research product.
You have already added 0 works in your ORCID record related to the merged Research product.
<script type="text/javascript">
<!--
document.write('<div id="oa_widget"></div>');
document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=undefined&type=result"></script>');
-->
</script>
Effect of lhcsr gene dosage on oxidative stress and light use efficiency by Chlamydomonas reinhardtii cultures

Unicellular green algae, a promising source for renewable biofuels, produce lipid-rich biomass from light and CO2. Productivity in photo-bioreactors is affected by inhomogeneous light distribution from high cell pigment causing heat dissipation of light energy absorbed in excess and shading of the deep layers. Contrasting reports have been published on the relation between photoprotective energy dissipation and productivity. Here, we have re-investigated the relation between energy quenching (qE) activity, photodamage and light use efficiency by comparing WT and two Chlamydomonas reinhardtii strains differing for their complement in LHCSR proteins, which catalyse dissipation of excitation energy in excess (qE). Strains were analysed for ROS production, protein composition, rate of photodamage and productivity assessed under wide light and CO2 conditions. The strain lacking LHCSR1 and knocked down in LHCSR3, thus depleted in qE, produced O2 at significantly higher rate under high light, accompanied by enhanced singlet oxygen release and PSII photodamage. However, biomass productivity of WT was delayed in respect for mutant strains under intermittent light conditions only, implying that PSII activity was not the limiting factor under excess light. Contrary to previous proposals, domestication of Chlamydomonas for carbon assimilation rate in photo-bioreactors by down-regulation of photoprotective energy dissipation was ineffective in increasing algal biomass productivity.
- University of Verona Italy
- University of Verona Italy
- University of Ferrara Italy
NPQ, Algae, Photoprotection, Gene Dosage, Light-Harvesting Protein Complexes, Cell growth, Oxidative Stress, Biomass, Photosynthesis, Algae, Biomass, Cell growth, NPQ, Photoprotection, Photosynthesis, Biomass, Chlamydomonas reinhardtii, Oxidative Stress, Light-Harvesting Protein Complexes, Gene Dosage, Chlamydomonas reinhardtii
NPQ, Algae, Photoprotection, Gene Dosage, Light-Harvesting Protein Complexes, Cell growth, Oxidative Stress, Biomass, Photosynthesis, Algae, Biomass, Cell growth, NPQ, Photoprotection, Photosynthesis, Biomass, Chlamydomonas reinhardtii, Oxidative Stress, Light-Harvesting Protein Complexes, Gene Dosage, Chlamydomonas reinhardtii
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).11 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
