Artificially mimicking aging of an equilibrium catalyst (ECAT) is an effective strategy to model the deactivation of a Fluid Catalytic Cracking (FCC) catalyst during refinery operations. Herein, we have used a correlative microscopy approach to unravel inter-particle spatial heterogeneities in artificially deactivated catalysts (DCATs) and compared them with a real-life ECAT containing on average 3800 ppm of Ni and 2300 ppm of V, and a set of density separated ECAT fractions. By doing so we could rationalize the effect of metal contaminants on catalyst acidity and pore accessibility. More specifically, the Fe, Ni, and V distributions were obtained using X-Ray Fluorescence (XRF), while Confocal Fluorescence Microscopy (CFM) after thiophene and Nile Blue A staining, respectively provided a visualization of Brønsted acid sites and accessibility distribution. We found that not only the metal poisons distribution, but also hydrothermal degradation, that affects ECATs dealumination and related acidity drop, need to be properly reproduced by artificial catalyst deactivation protocols. Fe contamination must also be taken into account since it affects matrix accessibility.