In this research, efforts were put to demonstrate synergistic interactions between bioenergy generation and wastewater treatment. The extent of such synergistic effect was assessed against wastewater effluents released from the beverage industry through the operation of a membrane-less truncated conical (TC) microbial fuel cell (MFC). A graphite-based reactor was operated for five cycles in batch mode using beverage industry wastewater as an organic substrate. Maximum bioelectricity produced on the fifth operating cycle corresponded to a voltage of 338 mV and a power of 1.14 mW at 100 Ω. The MFC recorded a higher substrate degradation rate (0.84 kg of chemical oxygen demand [COD]/m3-day) accompanied by the development of an electroactive biofilm and polarization behavior (e.g., a reduction in internal resistance from 323 Ω to 197 Ω over five operation cycles). Cyclic voltammetry showed a maximum performance of the biofilm during the fifth cycle (through its enrichment) as interpreted by oxidation and reduction currents of 2.48 and -2.21 mA, respectively. The performance of the proposed MFC was superior to other designs reported previously in both effluent treatment and bioenergy generation. A maximum treatment efficiency of 84.4% (in 385 h) was seen at an organic load (COD) of 3500 mg/L with the specific power yield (0.504 W/Kg of substrate (COD) removal) and volumetric power yield (15.03 W/m3). Our experimental studies support that the proposed system could be upscaled to realize the commercial operation.