Global warming and nitrogen (N) deposition have a profound impact on greenhouse gas (GHG) fluxes and consequently, they also affect climate change. However, the global combined effects of warming and N addition on GHG fluxes remain to be fully understood. To address this knowledge gap, a global meta-analysis of 197 datasets was performed to assess the response of GHG fluxes to warming and N addition and their interactions under various climate and experimental conditions. The results indicate that warming significantly increased CO2 emissions, while N addition and the combined warming and N addition treatments had no impact on CO2 emissions. Moreover, both warming and N addition and their interactions exhibited positive effects on N2O emissions. Under the combined warming and N addition treatments, warming was observed to exert a positive main effect on CO2 emissions, while N addition had a positive main effect on N2O emissions. The interactive effects of warming and N addition exhibited antagonistic effects on CO2, N2O, and CH4 emissions, with CH4 uptake dominated by additive effects. Furthermore, we identified biome and climate factors as the two treatments. These findings indicate that both warming and N addition substantially impact soil GHG fluxes and highlight the urgent need to investigate the influence of the combination of warming and N addition on terrestrial carbon and N cycling under ongoing global change.