Abstract Numerous studies have shown that architectural design affects energy performance significantly. However, the effect of space layouts on building energy performance has not been fully analysed. In this paper, we aim to study the effect of space layouts on energy performance. An office building was used as the reference, and 11 layout variants were proposed and compared for energy performance. Three climates (temperate, cold and tropical) were inspected, with three typical cities (Amsterdam, Harbin and Singapore). Dynamic simulation was conducted for the energy performance assessment integrating daylighting simulation with energy simulation. For each layout, two situations were simulated: one has no shading system, and the other one has an exterior screen for shading. Based on the simulation results, it is found that lighting demand is affected the most by the layout variance, and the resulting maximum difference (difference divided by the highest demand) happens in Harbin, being 46% without shading and 35% with shading. Regarding the sum of the final energy for heating, cooling and lighting, using a heat pump system , the maximum difference is 8% for the layouts both without and with shading system occurring in Amsterdam.