Biodiesel production from waste cooking oil is a viable alternative both to satisfy renewable energy demand and utilize a low-cost feedstock. However, a preparatory procedure is required to reduce free fatty acid contained in waste cooking oil to a specific value which is time- and energy-extensive. Herein, a fast and non-catalytic amidation reaction performs at room temperature is established as an alternative method to reduce the free fatty acid level. The effect of the molar ratio of waste cooking oil to monoethanolamine, reaction time and rotational speed of homogenizer were investigated on the conversion of free fatty acid to alkanolamide compounds. The highest conversion of 95.9 ± 0.02% was achieved in presence of a molar ratio of 1:1.5, reaction time of 0.5 min and rotational speed of 5000 rpm. Further, a homogenizer was used to facilitate the transesterification reaction using an alkaline catalyst at room temperature. The conversion of waste cooking oil to biodiesel of 91.4 ± 0.2% was achieved after 5 min of reaction time. In terms of processing parameters, the non-catalytic amidation and alkaline base catalytic transesterification reaction assisted by a homogenizer device is a time-saved process as it could save 92% and 94% of reaction time compared to one- and two-steps method and was performed in ambient condition.