To research the flow-induced vibration characteristics of the natural gas loop, unsteady numerical simulation is carried out on the loop calculation model under different ambient temperatures and different flow rates, and the influence of different flow rates on the dynamic characteristics of the flow field and its induced vibration characteristics is obtained. The results show that the inherent frequency of the natural gas loop increases slightly under the action of heat–fluid–solid coupling. The maximum equivalent stress of the loop increases with the increase in ambient temperature under low flow conditions, but it is almost constant under high flow conditions. The smaller the cross-sectional area of the loop pipeline inlet, the greater the pressure, and the more significant the pressure gradient along the flow direction. The pressure pulsation of monitoring points in the pipeline under different flow rates presents different rules, and the pulsation amplitude of pipes with different diameters is different, among which the amplitude of the pipe with a diameter of 250 mm is the largest and that of the pipe with a diameter of 150 mm is the smallest. The pressure pulsation signals are concentrated in the low-frequency band of 0–10 Hz, and the range of the band decreases with the increase in the flow rate. The vibration frequency of the loop structure is close to the fluid pressure pulsation frequency and the inherent frequency under the action of heat–fluid–solid coupling, which causes a resonance of about 2 Hz.