We have investigated electrical, charge transport and optical properties of functionalised DNA:PEDT-PSS thin films. Current-voltage dependencies of the samples were linear and symmetrical down to 77 K temperature. Material conductivity at room temperature was about (1–5)×10−10 Ω−1cm−1. The thermal activation energy of the conductivity measured in the dark was about 0.033 eV near the room temperature independently on the applied bias. The weakly expressed carrier trapping was identified by the Thermally Stimulated Current method, evidencing the fast recombination of light-generated carriers. Though, at constant light excitation a “bistable” photoconduction below the room temperature was identified. I.e., upon excitation by light from the spectral region ∼ 500 – 800 nm a notable increase of the photocurrent could be observed below 140 – 160 K by cooling the samples. Meanwhile by heating the photosensitivity remained increased up to 230 – 240 K. Most probably such phenomenon could be attributed to the light-induced morphology changes of the samples.