This paper presents a methodology to make EV able to contribute to secondary frequency regulation, by participating in Automatic Generation Control (AGC). The aggregator entity is used in this methodology to act as intermediate between the AGC and the EV controllers. The case-study used is an approximation of the Portuguese transmission/generation network, including existing tie lines with Spain, to which a solid three-phase short circuit was applied. After the disturbance, the impact of EV in the AGC operation was studied for two different situations: EV contributing to secondary frequency regulation and EV behaving as uncontrollable loads. The results obtained for both situations were then compared, in terms of interconnection active power flows in the existing tie-lines and its re-establishment time.