AbstractClimate warming is causing shifts in key life‐history events, including flowering time. To assess the impacts of increasing temperature on flowering phenology, it is crucial to understand the transcriptional changes of genes underlying the phenological shifts. Here, we conducted a comprehensive investigation of genes contributing to the flowering phenology shifts in response to increasing temperature by monitoring the seasonal expression dynamics of 293 flowering‐time genes along latitudinal gradients in the perennial herb, Arabidopsis halleri. Through transplant experiments at northern, southern and subtropical study sites in Japan, we demonstrated that the flowering period was shortened as latitude decreased, ultimately resulting in the loss of flowering opportunity in subtropical climates. The key transcriptional changes underlying the shortening of the flowering period and the loss of flowering opportunity were the diminished expression of floral pathway integrator genes and genes in the gibberellin synthesis and aging pathways, all of which are suppressed by increased expression of FLOWERING LOCUS C, a central repressor of flowering. These results suggest that the upper‐temperature limit of reproduction is governed by a relatively small number of genes that suppress reproduction in the absence of winter cold.