High surface area carbon supported bimetallic Pt-Fe and Pt-Ru alloy catalysts have been investigated for the oxygen electro- reduction and methanol oxidation , respectively, in low temperature direct methanol fuel cells (30-60 {degree sign}C). The cathode electrocatalyst was prepared by using a combination of colloidal and incipient wetness methods. For both anode and cathode catalysts, carbon supported bimetallic nanoparticles with particle size of 2-3 nm were obtained. These materials were studied in terms of structure, morphology and composition by using XRD, XRF and TEM techniques. The oxygen reduction and methanol oxidation processes were in-situ investigated in a DMFC device. For the oxygen reduction, the electrochemical data obtained with the Pt-Fe/C catalyst were compared to those obtained in the presence of a Pt/C catalyst characterised by the same concentration of active phase on carbon and similar particle size. An enhancement of the DMFC single cell performance was recorded with the Pt-Fe/C catalyst. It was observed that the ionomer loading play a significant role in determining the performance of the DMFC. The highest power density was recorded with electrodes containing 25% Nafion loading (~90 mW cm-2 at 60 {degree sign}C).