Cold-start (subzero startup) capability of polymer electrolyte fuel cell (PEFC) is of great importance. In this paper, the effects of the micro-porous-layer (MPL), varying start-up temperatures, start-up current density variation on the cold-start operation are investigated. We found PEFC with anode micro-porous-layer (AMPL), compared with one with CMPL, has higher possibility of successful cold start. By analyzing the anode and cathode pressure revolution of PEFC, the effect of MPL on the super cooled water removal and ice formation at different temperatures (-7,-10,-15 and-20℃) are discussed . In addition, we investigate the ice distribution in MEA through the X-ray device, by comparing the initial image when fuel cells shut down (before the produce the water) and final image after failed cold-start. We also explore the negative voltage phenomenon in the initial process of cold-start operation.