This study developed on-road exhaust emission and fuel consumption models for application in traffic microsimulations to estimate motorcycle emissions and fuel consumption in an Asian developing city. The motorcycle onboard measurement system was developed to instantaneously measure and continuously record on-road driving data, including the speed-time profile, exhaust emissions, and fuel consumption per second. The test motorcycle was driven on roads around Khon Kaen City, Thailand, to collect on-road driving data during the morning peak hours for a total of 112 hours. The collected on-road driving data were applied to develop on-road exhaust emission and fuel consumption models using regression analysis. The models were developed with high correlations among the amount of exhaust emissions and fuel consumption and the instantaneous speed and acceleration rate. The developed models were applied with a traffic microsimulation to evaluate the exclusive zone for motorcycles stopping at a signalized intersection. The evaluation results reveal that it could improve the level of intersection service by decreasing travel times, delays, and queue lengths at intersections, as well as by reducing the fuel consumption and emissions of vehicles travelling through intersections compared with these values under the existing conditions.