The desulfurization of biogas is essential for the successful operation of solid oxide fuel cells. H2S is one of the main components in biogas. In order to feed a solid oxide fuel cell, the contaminated gas has to be reduced to a certain degree. In this work, different parameters onto the desulfurization performance of commercially available desulfurization adsorbents were investigated. The experiments were carried out using a custom made lab-scale unit. Synthetic biogas was passed through the sorbent bed and the outlet H2S concentration was measured. Experimental runs in a fixed bed reactor were conducted to monitor H2S removal efficiency of a zinc oxide adsorbent, an adsorbent based on a mixture of manganese and copper oxide and a zeolite adsorbent. H2S removal efficiency was monitored under various operating conditions such as different temperatures, space velocities and inlet concentrations. This work provides useful data for adsorption tower design and process optimization.