AbstractThe widespread use of petroleum products in modern times has led to a search for alternative resources. Biofuel is a promising alternative to petroleum fuel, but biodiesel has a lower calorific value and is slightly more denser than diesel. To address this, a novel combination of GNA emulsified MME20 fuel is being investigated. This study aims to analyze the impact of a novel Nano additive blended biodiesel on engine performance and optimize the best compression ratio for the selected blend. The novelty of the study lies in the production of novel GNA emulsified MME fuel and its influence on a conventional CI engine. To achieve the objectives of the study, MME was produced using a two-phase transesterification method, and GNA was added to the MME20 at concentrations of 30, 60, and 90 ppm using the ultrasonication method. Engine experiments were then conducted using the prepared samples at CRs of 16, 17.5, and 19, and the results were compared with the standard diesel and MME20 blend. The results showed that the CP of the MME20 + GNA30 fuel at a CR 19 revealed a 14% increase compared to diesel. The ID of the fuel decreased by 20% compared to diesel at CR19, and there was a 23.5% increase in the CD for the MME20 + GNA30 blend compared to diesel at CR19. The BTHE for the MME20 + GNA30 fuel showed increases of 2.64% and BSFC and EGT decreases of 3.6% and 3.9%, respectively, at CR19 compared to the other blends. In summary, the study found that MME20 with GNA30, along with VCR, significantly enhanced the engine attributes compared to the pure diesel-operated standard CI engine conditions.