Disintegration of lignocellulosic biomass for energy purposes has been extensively studied. The study aimed to investigate the influence of crushed and uncrushed lignocellulosic biomass on biogas production in innovative reactor. The substrate fed to the reactor was Sida hermaphrodita silage mixed with cow manure. The bioreactor had innovative design mixing cage system. Mixing system of the bioreactor consisted of two cylindrical stirrers in the form of cage. The cages by rotation around the axis of the bioreactor at the same time turn against its own axis. The bioreactor is currently presented under the program Record Biomap (Horizon 2020). The bioreactor was operated at organic compounds loading 2 kg/(m3∙d) and 3 kg/(m3∙d) and hydraulic retention time was 50 d and 33 d, respectively. The biogas production under organic compounds loading 2 kg VS/(m3∙d) was 680 L/kg VS from crushed lignocellulosic biomass and 570 L/kg VS from uncrushed lignocellulosic biomass. The biogas production under organic compounds loading 3 kg VS/(m3∙d) was 730 L/kg VS from crushed lignocellulosic biomass and 630 L/kg VS from uncrushed lignocellulosic biomass. The crushing of substrate did not influence on methane content in the biogas. In all experiments about 54% of methane was in the biogas. The net energy efficiency was also calculated.