The present research is dedicated to the study of anaerobic co-digestion process of different biomass materials. Anaerobic co-digestion of digested sludge, grass silage, haylage and cattle manure was evaluated in mesophilic tank reactors in the lab-scale experiment. Twelve laboratory scale tank reactors (1.5 L) were used during the incubation period of 45 days. First triplet of reactors was fed with pure digested sludge and the other three with different mixtures having the volumetric ratios of 30/35/25/10, 40/30/20/10 and 50/25/15/10 for digested sludge/corn silage/grass haylage/cattle manure. Methane production was analyzed for all labscale reactors individually. The resulting specific methane production of above-mentioned batches was 336.34, 238.1 and 233.23 LSTP[CH4] kg-1 [TVS], respectively. Other results such as cumulative biogas and methane yield, volumetric biogas and methane yield, volumetric biogas and methane yield per day were also assessed. These results had the highest meaning when complex substrate had no more than 30% of inoculum