The aim of this study was to evaluate the application of the anaerobic bioreactor with fixed-structured bed (ABFSB) for brewery wastewater treatment with high volumetric organic loading rate (VOLR) and its comparison with a traditional packed-fixed bed bioreactor. Two different biomass support materials were tested, including polyurethane (PU) and polypropylene (PP) for both configurations. The best global efficiency was reached by the structured-fixed bed reactor with polyurethane as biomass support (SB PU). For a VOLR of 14.0 kg CODt m-3 d-1 (HRT of 8 h) and 20.3 kg CODt m-3 d-1(HRT of 12 h), the SB PU reached the average CODt removal efficiencies (ECOD) of 81% and 71%, respectively. The results show that ABFSB is a promising technology for high organic matter and solids concentration wastewater treatment, but the type of the biomass support had a big impact on the reactors performance.