Biohydrogen has significant feasibility since biological processes are much less energy intensive compared with electrolysis and thermo-chemical processes. It is widely recognized that considerable amounts of hydrogen (H2) can be produced from renewable resources without using energy from fossil fuels. Biological processes and bacterial fermentation are considered as the most environmentally friendly alternatives for satisfying future hydrogen demand. Biohydrogen production from agricultural and agro-industrial solid waste and wastewater is considered as highly advantageous as materials of this kind are abundant, cheap and biodegradable. The combustion of H2 with oxygen produces water as its only product: Unlike other fuels, the combustion of H2 does not produce carbon dioxide (CO2), carbon monoxide (CO), nitrogen oxides (NOx), sulfur dioxide (SO2), hydrocarbons or particulate matter (PM). Therefore, hydrogen is an environmentally friendly fuel where endeavors focus on producing specially designed internal combustion engines that can use H2 as fuel. The results showed that laser irradiated inoculum increased biohydrogen production by 1.2 times of the control. Therefore, in this research, it was hypothesized that exposing purple non-sulfur bacterial (PNSB) mix consortium to Helium-Neon red laser for 2 hours increased cell activity and consequently the biohydrogen production from food wastes through photo-fermentation process.