The double-layer diverging combustion chamber (DLDC chamber) aims to improve the fuel–air mixing formation and promote in-cylinder air utilization by changing fuel spray spreading characteristics. In order to investigate how the DLDC chamber profile and injection parameters affect the fuel spray spreading, visualization of fuel injection and impingement tests were carried out on two different DLDC chambers with different fuel injection parameters. The visualization test results showed that double-layer fuel spray spreading was obtained in the two DLDC chambers and the peripheral top clearance of each chamber was utilized efficiently. The DLDC chamber with a 50% upper layer volume provided a larger fuel spray distribution region after the start of injection. The DLDC chamber with a 70% upper layer volume obtained a larger fuel spray distribution region with better top clearance utilization at the later stage of injection. The injection parameters mentioned in this research showed significant effects on the fuel spray spreading in the DLDC chamber. Increasing the injection pressure provided a larger fuel spray distribution area at the beginning of injection. Decreasing the nozzle hole diameter had a positive influence on obtaining a larger fuel spray distribution. Advancing the injection timing enabled the enlarging of the fuel distribution region.